This case report illustrates a successful pregnancy and birth after vitrification of a human
blastocyst using modified cryostorage- Cryo E. A 31-year-old woman underwent short protocol for ovarian
hyperstimulation in an IVF treatment program. After administration of intramuscular hCG, all oocytes were
recovered transvaginally with ultrasound guidance. Twelve mature oocytes were obtained and eight were
fertilized. Three out of eight embryos became blastocyst, and two blastocysts were transferred on day 5, but no
implantation occurred. The other was cryopreserved by vitrification technique using a cryo E. Three months
after freezing, the cryopreserved blastocyst was thawed and survived. It was transferred to the patient’s uterus.
Blastocyst implantation resulted in a healthy single pregnancy. After continuing pregnancy until term, she
delivered by cesarean section due to unfavorable cervical conditions. A male baby with Apgar score 9/10,
weighing 3,500 g was born. Her child showed normal growth.

Keywords: Vitrification, Cryo E, Cryostorage, Blastocyst, Pregnancy