Background: Bacterial vaginosis (BV) is a disorder of the vaginal ecosystem characterized by a shift in the vaginal flora form the normally predominated lactobacillus to one dominated by sialidase enzymeproducing mixed flora.
Objectives: To compare the sensitivity of BVBLUE test for diagnosis of bacterial vaginosis with Gram stain by using Nugent score as a gold standard.
Material and Method: From April to June, 2004, a total of 173 pregnant women who received antenatal care at Phramongkutklao Hospital had reached the study criteria. The speculum for this exam, used in the process of collecting vaginal secretions, must not be lubricated with any lubricants. The vaginal discharge was collected from the lower 1/3 of the vaginal wall. Gram stain score and BVBLUE test were conducted and compared.
Results: 173 patients were enrolled in the present study. BVBLUE test was compared to the standard method for the diagnosis of BV by Gram stain using Nugent score as a gold standard. The sensitivity, specificity, accuracy, positive and negative predictive value of BVBLUE test versus the Gram stain score for diagnosis of bacterial vaginosis were 94%, 96%, 96% 86%, and 98%, respectively.
Conclusion: BVBLUE test for diagnosis of bacterial vaginosis had high sensitivity, specificity, accuracy, positive and negative predictive value (94%, 96%, 96%, 86%, and 98%, respectively).

Keywords: Bacterial vaginosis, Gram stain, BVBLUE test