Views: 1,590 | Downloads:
217
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
Mutation of ABO Gene in Thai Blood Donors with A3 Phenotype
Pipatvanichkul A 
,
Permpikul P ,
Vejbaesya S ,
Chinswangwatanakul W
,
Permpikul P ,
Vejbaesya S ,
Chinswangwatanakul W The ABO system is the most important of all blood group systems in transfusion practice. The subgroup gives a weak reaction when treated with anti-A or anti-B. The most common subgroup found in Thai blood donors is subgroup A3, which is characterized by mixed-field agglutination when reacted with anti-A and anti-A,B, was caused by mutation in the ABO gene, especially in the exon 7. In the present study mutation in A3 were charactered in exon 7 of the ABO gene in 10 A3 phenotype Thai blood donors from the National Blood Centre, Thai Red Cross Society by PCR amplification and DNA sequencing.
Mutations in exon 7 were identified in the A allele of six cases. In four cases, mutations were detected at positions 646T>A, 681G>A, 771C>T and 829G>A. One case showed a double mutations at positions 467C>T and 745C>T and one case showed a mutation at position 467C>T. Four cases showed wild type exon 7 as A101 allele. These mutations were previously reported in BGMUT database and no novel mutation was identified. These data suggest genetic heterogeneity in A3 phenotype in Thai blood donors.
Keywords: A3 phenotype, Blood group, ABO gene, Genetic mutation
Mutations in exon 7 were identified in the A allele of six cases. In four cases, mutations were detected at positions 646T>A, 681G>A, 771C>T and 829G>A. One case showed a double mutations at positions 467C>T and 745C>T and one case showed a mutation at position 467C>T. Four cases showed wild type exon 7 as A101 allele. These mutations were previously reported in BGMUT database and no novel mutation was identified. These data suggest genetic heterogeneity in A3 phenotype in Thai blood donors.
Keywords: A3 phenotype, Blood group, ABO gene, Genetic mutation
Download:
PDF