Material and Method: A cross-sectional study among the women who presented with an abnormal vaginal discharge (AVD) or asymptomatic annual cervical cytology screening was done. Each vaginal discharge was divided into two parts of investigation. The fi rst part included the clinical criteria of confi rmed BV, based on at least three out of fi ve indicators, the vaginal pH > 4.5, homogeneous and thin discharge (milky discharge), positive sniff/amine test, clue cell > 20% of total vaginal epithelial cells, and scanty or absence lactobacilli. The second part included the in-house preparation kit of suggestive BV, relied on elevated vaginal pH > 4.5 and positive amine tube test.

Results: Twenty-six women were enrolled. Of the complaint of AVD/asymptomatic had 2/10 of confi rmed BV (12 cases), and 1/13 of confi rmed non-BV (14 cases). The in-house preparation kit, compared with the clinical criteria, had sensitivity of 91%, specifi city of 71%, positive predictive value of 73%, and negative predictive value of 90%. There were false negative of 1/12 cases (8.3%), and false positive of 4/14 cases (28.5%).

Conclusion: The in-house preparation kit favorably compared with the clinical criteria and has the advantage of being simple, rapid, and easily performed in resource poor setting. Further development on sensitivity and specifi city of the test is suggested.

Keywords: Bacterial vaginosis, In-house, pH paper test, Amine tube test