Objective: Twenty one plants as ingredients of Thai traditional preparation called Sahasthara for relieve muscles pain and distal numbness were studied for their antioxidant and anti-inflammatory activities.
Material and Method: The extracts were obtained by maceration with 95% ethanol. They were tested for their antioxidant activity by DPPH scavenging assay and anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines using Griess reagent.
Results: The ethanolic extract of Terminalia chebula Retz. (Gall) showed the highest antioxidant activity (EC50 = 3.34 μg/ml). Its fruit extract also exhibited the most potent inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cells, followed by Atractylodes lancea and Baliospermum montanum (IC50 = 3.3, 9.7 and 12.6 μg/ml, respectively).
Conclusion: The results obtained for anti-inflammatory and antioxidant activities of these plants correspond with theirtraditional use for muscle pain and inflammatory-related diseases.

Keywords: Antioxidant, Nitric oxide inhibition assay, Anti-inflammation, Muscle pain, Sahasthara