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Original ArticleOpen Access
Lack of Mutagenicity of Stevioside and Steviol in Salmonella typhimurium TA 98 and TA 100
Klongpanichpak S 
,
Temcharoen P ,
Toskulkao C ,
Apibal S ,
Glinsukon T
,
Temcharoen P ,
Toskulkao C ,
Apibal S ,
Glinsukon T Stevioside, a sweet-tasting diterpene glycoside derived from Stevia rebaudiana, and
steviol, a product from enzymatic hydrolysis of stevioside, were tested for mutagenic activity
by the in vitro Ames test, a preincubation method, using Salmonella typhimurium TA 98 and TA
100 as the tester strains, either in the presence or absence of metabolic activating system derived
from the sodium phenobarbital and 5,6-benzoflavone pretreated liver S9 fractions from various
animal species including rat, mouse, hamster and guinea pig. Stevioside and steviol at the concentrations
up to 50 mg and 2 mg per plate, respectively showed no mutagenic effect on both tester
strains either in the presence or absence of metabolic activating system. However, at the high concentration
both stevioside and steviol showed some toxic effects on both tester strains. The toxic
effect was decreased in the presence of the metabolic activating system.
steviol, a product from enzymatic hydrolysis of stevioside, were tested for mutagenic activity
by the in vitro Ames test, a preincubation method, using Salmonella typhimurium TA 98 and TA
100 as the tester strains, either in the presence or absence of metabolic activating system derived
from the sodium phenobarbital and 5,6-benzoflavone pretreated liver S9 fractions from various
animal species including rat, mouse, hamster and guinea pig. Stevioside and steviol at the concentrations
up to 50 mg and 2 mg per plate, respectively showed no mutagenic effect on both tester
strains either in the presence or absence of metabolic activating system. However, at the high concentration
both stevioside and steviol showed some toxic effects on both tester strains. The toxic
effect was decreased in the presence of the metabolic activating system.
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