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Original ArticleOpen Access
Immunopharmacological Activity of Polysaccharide from the Pericarb of Mangosteen Garcinia : Phagocytic Intracellular Killing Activities
Chanaral P 
,
Chanaral N ,
Fujihara M ,
Nagumo T
,
Chanaral N ,
Fujihara M ,
Nagumo T Polysaccharides from the pericarbs of mangosteen, Garcinia mangostana Linn., was
obtained by treating the dried ground pericarbs with hot water followed by ethanol precipitation
(M fraction). The extract was fractionated by anion exchange chromatography on a DEAE-cellulose
column as MDE1-5 fractions. The fractions of MDE3 and MDE4 composed of mainly
D-galacturonic acid and a small amount of neutral sugar (L-arabinose as the major one and Lrhamnose
and D-galactose as the minor ones) were studied for immunopharmacological activities
by phagocytic test to intracellular bacteria (Salmonella enteritidis) and nitroblue tetrazolium (NBT)
and superoxide generation tests. The results showed that the number of S. enteritidis in cultured
monocyte with extract of pericarb of mangosteen (MDE3) was killed. Activating score (mean± SD)
of NBT test of 100 polymorphonuclear phagocytic cells were 145±78, 338±58, 222±73, 209±77,
211±63, 372±19, 369±20, 355±34 in normal saline control, phorbol myristate acetate (PMA), MDE3,
MDE4, indomethacin (I), PMA + MDE3, PMA + MDE4 and PMA + I, respectively. Superoxide
generation test was also done by color reduction of cytochrome c. Both MDE3 and MDE4 stimulate
superoxide production. The number of S. enteritidis in cultured monocyte with extract of
pericarb of mangosteen was killed. This paper suggests that polysaccharides in the extract can
stimulate phagocytic cells and kill intracellular bacteria (S. enteritidis).
obtained by treating the dried ground pericarbs with hot water followed by ethanol precipitation
(M fraction). The extract was fractionated by anion exchange chromatography on a DEAE-cellulose
column as MDE1-5 fractions. The fractions of MDE3 and MDE4 composed of mainly
D-galacturonic acid and a small amount of neutral sugar (L-arabinose as the major one and Lrhamnose
and D-galactose as the minor ones) were studied for immunopharmacological activities
by phagocytic test to intracellular bacteria (Salmonella enteritidis) and nitroblue tetrazolium (NBT)
and superoxide generation tests. The results showed that the number of S. enteritidis in cultured
monocyte with extract of pericarb of mangosteen (MDE3) was killed. Activating score (mean± SD)
of NBT test of 100 polymorphonuclear phagocytic cells were 145±78, 338±58, 222±73, 209±77,
211±63, 372±19, 369±20, 355±34 in normal saline control, phorbol myristate acetate (PMA), MDE3,
MDE4, indomethacin (I), PMA + MDE3, PMA + MDE4 and PMA + I, respectively. Superoxide
generation test was also done by color reduction of cytochrome c. Both MDE3 and MDE4 stimulate
superoxide production. The number of S. enteritidis in cultured monocyte with extract of
pericarb of mangosteen was killed. This paper suggests that polysaccharides in the extract can
stimulate phagocytic cells and kill intracellular bacteria (S. enteritidis).
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