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Original ArticleOpen Access
Control Cells for Both Electrical Impedance and Light Scattering Automated Hematological Analyzers: Preparation from Normal and Thalassemic Blood Samples
Arnutti P 
,
Bunyaratavej A ,
Patanapanyasat K ,
Chuansumrit A
,
Bunyaratavej A ,
Patanapanyasat K ,
Chuansumrit A The study on quality control of automated blood cell analyzers, Technicon H* 1 and
Coulter MAXM by using three separately self-prepared control cells was extensively investigated.
The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by glutaraldehyde,
and control red cells from normal and thalassemic patients preserved and anticoagulated
in CPO or CPDA-1. The Technicon H*l system was based on the principle of light
scattering but the Coulter MAXM was based on the principle of electrical impedance for cell
counting and measurement. The self-prepared control cells can be satisfactorily utilized as control
for each system with statistically significant difference (p < 0.05) for both systems. The expirated
dates for control cells are different in both systems and should be determined for each system specifically.
The control red cells prepared from thalassemic patients were quite satisfactorily useful
as an abnormal control for both systems during this study.
Coulter MAXM by using three separately self-prepared control cells was extensively investigated.
The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by glutaraldehyde,
and control red cells from normal and thalassemic patients preserved and anticoagulated
in CPO or CPDA-1. The Technicon H*l system was based on the principle of light
scattering but the Coulter MAXM was based on the principle of electrical impedance for cell
counting and measurement. The self-prepared control cells can be satisfactorily utilized as control
for each system with statistically significant difference (p < 0.05) for both systems. The expirated
dates for control cells are different in both systems and should be determined for each system specifically.
The control red cells prepared from thalassemic patients were quite satisfactorily useful
as an abnormal control for both systems during this study.
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