J Med Assoc Thai 1998; 81 (8):565

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Comparison of the Fertilization Rate after Intracytoplasmic Sperm Injection (ICSI) Using Ejaculated Sperms, Epididymal Sperms and Testicular Sperms
Sukcharoen N Mail, Sithipravej T , Chinpilas V , Boonkasemsanti W

Intracytoplasmic sperm injection (ICSI) has been successfully used to achieve fertilization
and pregnancies for patients with extreme oligoastheno-zoospermia using ejaculated
sperms or patients with azoospermia using epididymal or testicular sperms. The aim of this study
was to compare the fertilization rate after ICSI using ejaculated, epididymal and testicular
sperms. Between January and September 1997, 10 azoospermic men underwent percutaneous
epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE) to recover sperm for
ICSI. A total of 5 PESA cases and 5 TESE cases were performed at the Center for Assisted Reproduction
& Embryology. Thirty-one patients performed ICSI using ejaculated sperms during the
same period of time were used as a control group. ICSI using ejaculated sperms, epididymal
sperms from PESA and testicular sperms from TESE was a highly successful technique,
achieving fertilization rates of 78.5 per cent, 83.3 per cent and 80.8 per cent, respectively. Good
fertilization rates were achieved without significant differences among the various sperm

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