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Original ArticleOpen Access
Comparison of Two-layer Percoll Gradient and Mini-Percoll Methods for Sperm Preparation
Kunathikom S 
,
Ko-Anantakul S ,
Techatraisak K ,
Treetampinich C ,
Phophong P ,
Karavagul C
,
Ko-Anantakul S ,
Techatraisak K ,
Treetampinich C ,
Phophong P ,
Karavagul C To compare the efficiency of sperm preparation between the two-layer Percoll gradient
and mini-Percoll methods, 50 normal and 33 abnormal semen samples from male partners of
infertile couples were studied. The number of recovered spermatozoa, percentage of motility,
percentage of normal morphology, and their survival at 24 and 48 hours were assessed. Both
Percoll gradient techniques resulted in a significantly higher percentage of motility and percentage
of normal morphology compared with the original semen samples (p
and mini-Percoll methods, 50 normal and 33 abnormal semen samples from male partners of
infertile couples were studied. The number of recovered spermatozoa, percentage of motility,
percentage of normal morphology, and their survival at 24 and 48 hours were assessed. Both
Percoll gradient techniques resulted in a significantly higher percentage of motility and percentage
of normal morphology compared with the original semen samples (p
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