J Med Assoc Thai 1999; 82 (11):1142

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Effects of Different Biopsy Methods on the Development of Preimplantation Mouse Embryos, in vitro and in vivo : Implication for Preimplantation Genetic Diagnosis
Tangchaisin P Mail, Pruksananonda K , Tirawamapong T , Yodyingyuad V , Viratamasen P

The aim of this study was to investigate the efficacy of two different embryo biopsy
techniques, direct aspiration and partial zona dissection (PZD)-push, on subsequent in vitro
and in- vivo development of 8-cell stage mouse embryos. It was found that the rates of normal
blastocyst formation and hatching blastocysts of direct aspiration, PZD-push, solution control
and control embryos were not significantly different (80.8%, 81.6%, 84.5%, 86.7% and
71.9%, 72.3% and 74.6%) respectively. There was, however, a significant reduction in rate of
complete hatching blastocysts (P

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