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Original ArticleOpen Access
p53 Gene Mutations in Non-Small Cell Lung Cancer from Thai Patients
Petmitr S 
,
Makthiengtrong S ,
Sutinont P ,
Migasena P
,
Makthiengtrong S ,
Sutinont P ,
Migasena P Paraffin embedded tissues from twenty-two Thai patients with non-small cell lung
cancer were studied for p53 gene mutations in exon 5 to 8 using polymerase chain reaction and
single-stranded conformation polymorphism (PCR-SSCP) followed by thermal cycle sequencing.
Results showed that point mutations in this region of p53 gene were present in 3 cases. One
harboured the base change from GAC to AAC at codon 281, changing amino acid from aspartic
acid to asparagine, whilst the other two cases were transversion of AAA (lysine) to ACA (threonine)
at codon 292. All subjects with p53 mutation had a past history of tobacco smoking.
Key word : p53 Gene, Lung Cancer, Thai Patients
cancer were studied for p53 gene mutations in exon 5 to 8 using polymerase chain reaction and
single-stranded conformation polymorphism (PCR-SSCP) followed by thermal cycle sequencing.
Results showed that point mutations in this region of p53 gene were present in 3 cases. One
harboured the base change from GAC to AAC at codon 281, changing amino acid from aspartic
acid to asparagine, whilst the other two cases were transversion of AAA (lysine) to ACA (threonine)
at codon 292. All subjects with p53 mutation had a past history of tobacco smoking.
Key word : p53 Gene, Lung Cancer, Thai Patients
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