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Original ArticleOpen Access
Detection of HBV Genome by Gene Amplification Method in HBsAg Negative Blood Donors
Bodhiphala P 
,
Chaturachwnroenchai S ,
Pruksananonda P
,
Chaturachwnroenchai S ,
Pruksananonda P The incidence of post-transfusion hepatitis has been reduced greatly by screening blood
donors for hepatitis B surface antigen (HBsAg). However, hepatitis B virus infection still accounts
for a certain number of cases of post-transfusion hepatitis. The purpose of this study was to detect
HBV DNA in the HBsAg negative blood samples by using nested PCR with two primer pairs specific
to core region. Two hundreds blood samples from HBsAg negative donors, and 14 samples
from HBsAg positive donors were provided by the blood bank of Ramathibodi Hospital. The results
showed that HBV DNA was detected in all 14 HBsAg positive blood samples and in 7 (3.5o/c) of
200 HBsAg negative blood samples.
This study showed that the absence of HBsAg in otherwise apparently healthy individuals
may not be enough to ensure lack of circulating HBV. The more sensitive ELISA technique is still
in need. Otherwise, the safety of blood transfusion can be enhanced by careful selection of blood
donors and careful consideration of risks and benefit of the patients who need blood transfusions.
Key word : HBsAg, PCR, Blood Donor, HBV DNA, ELISA
donors for hepatitis B surface antigen (HBsAg). However, hepatitis B virus infection still accounts
for a certain number of cases of post-transfusion hepatitis. The purpose of this study was to detect
HBV DNA in the HBsAg negative blood samples by using nested PCR with two primer pairs specific
to core region. Two hundreds blood samples from HBsAg negative donors, and 14 samples
from HBsAg positive donors were provided by the blood bank of Ramathibodi Hospital. The results
showed that HBV DNA was detected in all 14 HBsAg positive blood samples and in 7 (3.5o/c) of
200 HBsAg negative blood samples.
This study showed that the absence of HBsAg in otherwise apparently healthy individuals
may not be enough to ensure lack of circulating HBV. The more sensitive ELISA technique is still
in need. Otherwise, the safety of blood transfusion can be enhanced by careful selection of blood
donors and careful consideration of risks and benefit of the patients who need blood transfusions.
Key word : HBsAg, PCR, Blood Donor, HBV DNA, ELISA
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