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Original ArticleOpen Access
Development of Coagglutination Reagents for Serological Grouping of Streptococci
Hanvivatvong O 
,
Sirilertpana S ,
Chuticlrop C ,
Tirawatnapong S
,
Sirilertpana S ,
Chuticlrop C ,
Tirawatnapong S Coagglutination reagents for the rapid serological grouping of groups A, B, C, F and G
Streptococcus
have been developed. Antisera to groups A, B, C, F and G
Streptococcus
were raised
in rabbits. After absorption with cross-reacting antigens, the specific antibodies were coated on
Staphylococcus
protein-A and used as group-specific coagglutination reagents. The sensitivity of
the reagents for groups A, C and G
Streptococcus
was 100 per cent and the specificity was I 00,
100, and 98.77 per cent, respectively. The sensitivity and specificity of these reagents were con-
sistent up to 12 months, although specificity declined with longer storage. The in-house coagglu-
tination reagents for groups A, C and G streptococcus were also tested in comparison with the
commercially available
Streptococcus
PhadebactเธŽ test and yielded almost identical results. Sensi-
tivity of the in-house of group B
Streptococcus
reagent was low, while the group F reagent gave
a high incidence of false positive reaction.
Key word : Coagglutination, Streptococci
Streptococcus
have been developed. Antisera to groups A, B, C, F and G
Streptococcus
were raised
in rabbits. After absorption with cross-reacting antigens, the specific antibodies were coated on
Staphylococcus
protein-A and used as group-specific coagglutination reagents. The sensitivity of
the reagents for groups A, C and G
Streptococcus
was 100 per cent and the specificity was I 00,
100, and 98.77 per cent, respectively. The sensitivity and specificity of these reagents were con-
sistent up to 12 months, although specificity declined with longer storage. The in-house coagglu-
tination reagents for groups A, C and G streptococcus were also tested in comparison with the
commercially available
Streptococcus
PhadebactเธŽ test and yielded almost identical results. Sensi-
tivity of the in-house of group B
Streptococcus
reagent was low, while the group F reagent gave
a high incidence of false positive reaction.
Key word : Coagglutination, Streptococci
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