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Original ArticleOpen Access
Genotyping of Human Platelet Antigens in Ethnic Northeastern Thais by the Polymerase Chain Reaction-Sequence Specific Primer Technique
Romphruk AV 
,
Akahat J ,
Srivanichrak P ,
Puapairoj C ,
Roomphruk A ,
Leelayuwat C
,
Akahat J ,
Srivanichrak P ,
Puapairoj C ,
Roomphruk A ,
Leelayuwat C JONGKOL AKAHAT, M.Sc.*,
CHINTANA PUAPAIROJ, M.D.*,
CHANVIT LEELAYUWAT, Ph.D.**
Human platelet antigens (HPA) are important in neonatal alloimmune thrombocytopenia
(NAITP), post-transfusion purpura (PTP), refractoriness to platelet transfusion therapy and
population genetics. Th_e distribution of HPA in a Northeast Thai population was studied. 300
healthy, unrelated, and ethnic Northeastern Thais were randomly selected. Using the polymerase
chain reaction-sequence specific primer technique (PCR-SSP), the frequency of HPA-1, -2, -3,
-4, -5 and -6 were determined. The phenotype frequencies were 100 per cent for HPA-la, 4a, 5a,
and 6a. For HPA-lb, 2a, 2b, 3a, 3b, 5b and 6b, the frequencies were 5.7, 99.7, 12.3, 78.0, 71.3,
7.3 and 3.0 per cent, respectively. The HPA-4b was not found. The HPA frequencies in our sub-
jects were quite similar to other Asian populations but were different from Caucasians. The
distribution of HPA genotypes encountered in our study indicate that HPA-la, -4a, -4b, -5a and -6a
will not be involved in NAITP, PTP and refractoriness to platelet transfusion therapy in North-
eastern Thais. Moreover, HPA-lb, -2a, -2b, -3a, -3b, -5b and -6b may induce alloantibodies in
these patients.
Key word : Platelet Antigens, PCR-SSP
CHINTANA PUAPAIROJ, M.D.*,
CHANVIT LEELAYUWAT, Ph.D.**
Human platelet antigens (HPA) are important in neonatal alloimmune thrombocytopenia
(NAITP), post-transfusion purpura (PTP), refractoriness to platelet transfusion therapy and
population genetics. Th_e distribution of HPA in a Northeast Thai population was studied. 300
healthy, unrelated, and ethnic Northeastern Thais were randomly selected. Using the polymerase
chain reaction-sequence specific primer technique (PCR-SSP), the frequency of HPA-1, -2, -3,
-4, -5 and -6 were determined. The phenotype frequencies were 100 per cent for HPA-la, 4a, 5a,
and 6a. For HPA-lb, 2a, 2b, 3a, 3b, 5b and 6b, the frequencies were 5.7, 99.7, 12.3, 78.0, 71.3,
7.3 and 3.0 per cent, respectively. The HPA-4b was not found. The HPA frequencies in our sub-
jects were quite similar to other Asian populations but were different from Caucasians. The
distribution of HPA genotypes encountered in our study indicate that HPA-la, -4a, -4b, -5a and -6a
will not be involved in NAITP, PTP and refractoriness to platelet transfusion therapy in North-
eastern Thais. Moreover, HPA-lb, -2a, -2b, -3a, -3b, -5b and -6b may induce alloantibodies in
these patients.
Key word : Platelet Antigens, PCR-SSP
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