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Original ArticleOpen Access
Measurement of Serum Free IGF-1 in Diagnosis of Growth Hormone Deficiency
Wacharasindhu S 
,
Chaichanwatanakul K ,
Likitmaskul S ,
Angsusingha K
,
Chaichanwatanakul K ,
Likitmaskul S ,
Angsusingha K Serum levels of total insulin-like growth factor-! (IGF-1) are growth hormone (GH)
dependent and can be used as the screening tool for GH deficient status. However, most of them
are bound to IGF-binding proteins, leaving less than 1 per cent in the free or unbound forms
which represent the active biological fractions. Serum free IGF-I levels were measured by
radioimmunoassay (IRMA) in 48 short children with various conditions. We found that the
means
เธ‘
SEM of free IGF-I in children with panhypopituitarism (PAN) and complete growth
hormone deficiencies (cGHD) were significantly lower than those in sex and age matched
normal children (0.02 เธ‘ 0.01
vs
2.01 เธ‘ 0.7 ng/ml, p
=
0.0006 and 0.42 เธ‘ 0.18
vs
1.72 เธ‘ 0.27 ng/ml,
p
=
0.0007 respectively) but not in children with partial growth hormone deficiencies (pGHD)
(0.91 เธ‘ 0.3
vs
1.97 เธ‘ 0.4 ng/ml, p
=
0.27) and idiopathic short stature (ISS) (0.94 เธ‘ 0.3
vs
1.95 เธ‘ 0.6
ng/ml, p
=
0.13). However, when we classified the pGHD children into 2 groups according to
IGFBP-3 SDS for normal Thai children, we found that the mean of free IGF-I levels in pGHD
children with IGFBP-3 SDS
~
-1.3 was significantly lower than that of the controls. (0.68
เธ‘
0.55
vs
2.66 เธ‘ 0.71 ng/ml, p
=
0.04)
In conclusion, the measurement of free IGF-I level can be used to evaluate the GH status
of short children and might be used as a guide when starting treatment.
Key word : Insulin-like Growth Factor-!
dependent and can be used as the screening tool for GH deficient status. However, most of them
are bound to IGF-binding proteins, leaving less than 1 per cent in the free or unbound forms
which represent the active biological fractions. Serum free IGF-I levels were measured by
radioimmunoassay (IRMA) in 48 short children with various conditions. We found that the
means
เธ‘
SEM of free IGF-I in children with panhypopituitarism (PAN) and complete growth
hormone deficiencies (cGHD) were significantly lower than those in sex and age matched
normal children (0.02 เธ‘ 0.01
vs
2.01 เธ‘ 0.7 ng/ml, p
=
0.0006 and 0.42 เธ‘ 0.18
vs
1.72 เธ‘ 0.27 ng/ml,
p
=
0.0007 respectively) but not in children with partial growth hormone deficiencies (pGHD)
(0.91 เธ‘ 0.3
vs
1.97 เธ‘ 0.4 ng/ml, p
=
0.27) and idiopathic short stature (ISS) (0.94 เธ‘ 0.3
vs
1.95 เธ‘ 0.6
ng/ml, p
=
0.13). However, when we classified the pGHD children into 2 groups according to
IGFBP-3 SDS for normal Thai children, we found that the mean of free IGF-I levels in pGHD
children with IGFBP-3 SDS
~
-1.3 was significantly lower than that of the controls. (0.68
เธ‘
0.55
vs
2.66 เธ‘ 0.71 ng/ml, p
=
0.04)
In conclusion, the measurement of free IGF-I level can be used to evaluate the GH status
of short children and might be used as a guide when starting treatment.
Key word : Insulin-like Growth Factor-!
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