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Original ArticleOpen Access
Timing of Second Polar Body Extrusion and Pronuclear Formation After Intracytoplasmic Sperm Injection (ICSI)
Suppinyapang S 
,
Chaavaratana R ,
Karavakul C
,
Chaavaratana R ,
Karavakul C To determine timing of second polar body extrusion and pronuclear formation to find
out when the intracytoplasmic sperm injected (ICSI) oocytes should be checked for successful
fertilization.148 oocytes from 15 patients from January to May 1998 were analyzed. Metaphase
II oocytes were followed by ICSI. Fertilization was checked at 2, 4, 6, 8, 16 and 18 hours later for
observation of second polar body extrusion and pronuclear formation. Normal fertilization was
achieved in 73.6 per cent of the oocytes (1091148). After ICSI at 2, 4, 6, 8, 16 and 18 hours, extru-
sion of second polar body was 8.3 per cent, 49.5 per cent, 84.4 per cent, 98.2 per cent, 98.2
per cent, 98.2 per cent and pronuclear formation was 0 per cent, 0 per cent, 8.3 per cent, 30.3
per cent, 96.3 per cent, 100 per cent respectively. The earliest extrusion of second polar body
and pronuclear formation were at 2 and 6 hours after ICSI respectively. This study suggests
that the appropriate time to determine fertilization is at 2, 6 and 18 hours after ICSI.
Key word : Second Polar Body Extrusion, Pronuclear Formation, Intracytoplasmic Sperm Injection
out when the intracytoplasmic sperm injected (ICSI) oocytes should be checked for successful
fertilization.148 oocytes from 15 patients from January to May 1998 were analyzed. Metaphase
II oocytes were followed by ICSI. Fertilization was checked at 2, 4, 6, 8, 16 and 18 hours later for
observation of second polar body extrusion and pronuclear formation. Normal fertilization was
achieved in 73.6 per cent of the oocytes (1091148). After ICSI at 2, 4, 6, 8, 16 and 18 hours, extru-
sion of second polar body was 8.3 per cent, 49.5 per cent, 84.4 per cent, 98.2 per cent, 98.2
per cent, 98.2 per cent and pronuclear formation was 0 per cent, 0 per cent, 8.3 per cent, 30.3
per cent, 96.3 per cent, 100 per cent respectively. The earliest extrusion of second polar body
and pronuclear formation were at 2 and 6 hours after ICSI respectively. This study suggests
that the appropriate time to determine fertilization is at 2, 6 and 18 hours after ICSI.
Key word : Second Polar Body Extrusion, Pronuclear Formation, Intracytoplasmic Sperm Injection
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