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Original ArticleOpen Access
p53 Protein Expression in Cutaneous Squamous Cell Carcinoma
Auepemkiate S 
,
Sriplung H
,
Sriplung H Background: p53 is a nucleoprotein encoded by a tumor suppressor gene. It's mutations
are implicated in the genesis of a wide variety of malignant neoplasia including skin cancers.
Objective: To study the expression of the p53 protein in cutaneous squamous cell car-
cinoma (SCCs) and evaluate the relationships between this expression and sites, varying degrees
of differentiation and amounts of apoptotic cells.
Method: Sixty-seven tissue samples of SCCs from Songklanagarind Hospital obtained
from January 1991 to December 1996 were examined by immunohistochemistry using polyclonal
anti p53-CM1. (Novocastra Laboratories, Newcastle, England, dilution 1 :700)
Result: p53 Immunoreactivity was demonstrated in 26.87 per cent of SCCs. This was
observed in 15/51 of sun-exposed cases and 3/16 of sun-protected cases (p
=
0.401). The more
differentiated the tumor, the less p53 staining was observed (p
=
0.043). There was no association
between p53 positivity and the amounts of apoptotic cells.
Conclusion: The p53 expression is not related to the sun exposure.
It
does not represent
a commitment to apoptosis. However, it may indicate the differentiation and/or proliferative status
of the tumor cells.
Key word : p53, Cutaneous Squamous Cell Carcinoma
are implicated in the genesis of a wide variety of malignant neoplasia including skin cancers.
Objective: To study the expression of the p53 protein in cutaneous squamous cell car-
cinoma (SCCs) and evaluate the relationships between this expression and sites, varying degrees
of differentiation and amounts of apoptotic cells.
Method: Sixty-seven tissue samples of SCCs from Songklanagarind Hospital obtained
from January 1991 to December 1996 were examined by immunohistochemistry using polyclonal
anti p53-CM1. (Novocastra Laboratories, Newcastle, England, dilution 1 :700)
Result: p53 Immunoreactivity was demonstrated in 26.87 per cent of SCCs. This was
observed in 15/51 of sun-exposed cases and 3/16 of sun-protected cases (p
=
0.401). The more
differentiated the tumor, the less p53 staining was observed (p
=
0.043). There was no association
between p53 positivity and the amounts of apoptotic cells.
Conclusion: The p53 expression is not related to the sun exposure.
It
does not represent
a commitment to apoptosis. However, it may indicate the differentiation and/or proliferative status
of the tumor cells.
Key word : p53, Cutaneous Squamous Cell Carcinoma
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