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Original ArticleOpen Access
Modification of the Acetylcholinesterase (AchE) Staining Method in Hirschsprung's Disease
Chentanez V 
,
Chittmittrapap S ,
Kasantikul V
,
Chittmittrapap S ,
Kasantikul V Demonstration of the increasing activity of acetylcholinesterase (AChE) in a segment
of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung เธ— s
disease. Two methods of histochemical assessment were tried to establish the most appropriate
and effective method for this study within the limitation of available equipment. Lakeเธ— s method
was chosen and was modified as the standard histochemical examination.
Key word : Acetylcholiesterase Staining, Rectal Suction Biopsy, Hirschsprung's Disease
of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung เธ— s
disease. Two methods of histochemical assessment were tried to establish the most appropriate
and effective method for this study within the limitation of available equipment. Lakeเธ— s method
was chosen and was modified as the standard histochemical examination.
Key word : Acetylcholiesterase Staining, Rectal Suction Biopsy, Hirschsprung's Disease
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