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Original ArticleOpen Access
Semiquantitative Determination of Urinary Glucose : Comparison of Home-Made Strip and Routine Tests
Koonakosit R 
Glucose oxidase paper strips for semiquantitive determination of glucose in urine are com-
mercially available but details of their preparation are not published. The purpose of this study
was to produce such strips, using a coupled enzyme glucose oxidase-peroxidase reaction which
would yield purple color with ortho-tolidine, and safranin upon dipping into urine containing
glucose. In this present study, without any special equipment, special humidity and temperature
control, the new reagent strip named R-strip could be successfully prepared in the atmospheric
conditions of Thailand. R-strips were evaluated against random urine added with various amounts
of glucose in comparison with a commercial strip (T-strip), Benedict's test, and a commercial
tablet (C-tablet), routinely used in laboratories. The developed strips were found to be as specific
as T-strip and more sensitive than other tests.
Key
word
: Semiquantitative Test Strip, Urinary Glucose, Glucose Oxidase Test
mercially available but details of their preparation are not published. The purpose of this study
was to produce such strips, using a coupled enzyme glucose oxidase-peroxidase reaction which
would yield purple color with ortho-tolidine, and safranin upon dipping into urine containing
glucose. In this present study, without any special equipment, special humidity and temperature
control, the new reagent strip named R-strip could be successfully prepared in the atmospheric
conditions of Thailand. R-strips were evaluated against random urine added with various amounts
of glucose in comparison with a commercial strip (T-strip), Benedict's test, and a commercial
tablet (C-tablet), routinely used in laboratories. The developed strips were found to be as specific
as T-strip and more sensitive than other tests.
Key
word
: Semiquantitative Test Strip, Urinary Glucose, Glucose Oxidase Test
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