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Original ArticleOpen Access
Paternity Testing by PCR-Based STR Analysis
Rerkamnuaychoke B 
,
Chantratita W ,
Jomsawat U ,
Thanakitgosate J ,
Rojanaswwn P
,
Chantratita W ,
Jomsawat U ,
Thanakitgosate J ,
Rojanaswwn P WASUN CHANTRATITA, Ph.D.**,
JANPEN THANAKITGOSATE, B.Sc. *,
We present application of polymerase chain reaction (PCR) -based short tandem repeat
(STR) system for use in paternity testing. The process involves a single tube multiplex PCR of 9
STR loci on different chromosomes, in conjunction with Amelogenin sex test and internal size
standards, followed by using an automated DNA sequencer to detect amplified products. The
results showed that this system provided unambiguously reliable results. In addition, the method is
useful for routine use in that it is robust and reproducible and provides a reliable means of pater-
nity testing.
Key word : Paternity Testing, PCR, STR
JANPEN THANAKITGOSATE, B.Sc. *,
We present application of polymerase chain reaction (PCR) -based short tandem repeat
(STR) system for use in paternity testing. The process involves a single tube multiplex PCR of 9
STR loci on different chromosomes, in conjunction with Amelogenin sex test and internal size
standards, followed by using an automated DNA sequencer to detect amplified products. The
results showed that this system provided unambiguously reliable results. In addition, the method is
useful for routine use in that it is robust and reproducible and provides a reliable means of pater-
nity testing.
Key word : Paternity Testing, PCR, STR
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