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Original ArticleOpen Access
BCR/ABL Rearrangement in Philadelphia Chromosome Negative CML Patients
Rerkamnuaychoke B 
,
Jomsawat U ,
Maneesri N ,
Apilugsanachit A ,
Pattanasak N
,
Jomsawat U ,
Maneesri N ,
Apilugsanachit A ,
Pattanasak N Reverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients
with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromo-
some. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%)
cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases,
the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was
25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evi-
dence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested
PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative
CML patients.
Key word : BCR/ABL, Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Chronic
Myelogenous Leukemia (CML)
with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromo-
some. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%)
cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases,
the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was
25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evi-
dence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested
PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative
CML patients.
Key word : BCR/ABL, Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Chronic
Myelogenous Leukemia (CML)
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