J Med Assoc Thai 2000; 83 (11):19

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Polymorphism of the Gene Encoding Lipoprotein Lipase in Thai Primary Hyperlipoproteinemias
Tirawanchai N Mail, Dulyasukdi B , Likidlilid A , Pongrapeeporn K , Poldee S , Amomrallana A

Lipoprotein lipase (LPL) plays a central role in the clearance of very low density lipo-
protein (VLDL) and chylomicrons from the circulation. It also affects the maturation of high density
lipoprotein (HDL) and low density lipoprotein (LDL). LPL is an important candidate gene in
determining the risk factor in metabolic disorders including primary hyperlipidemia. Our study is
the first report from Thailand on the characterization of two common DNA polymorphisms, i.e
II and
III at introns 6 and 8, respectively of the LPL gene in 94 Thai dyslipidemic subjects
compared to 32 normolipidemic subjects using PCR-RFLP. It was observed that the frequencies
of the cut and uncut alleles of
II were 0.67 and 0.33 in normolipidemic subjects. Such fre-
quencies were 0.64 and 0.36 in hyperlipidemic subjects. Additionally, the frequencies of the cut
and uncut alleles of
III were found to be 0.73 and 0.27 in normolipidemic subjects. They
were 0.85 and 0.15 in hyperlipidemic subjects. The allele frequencies of the
III but not
II polymorphism in hyperlipidemic subjects were significantly different from normolipidemic
subjects (p0.05).
Key word : Lipoprotein Lipase, DNA Polymorphism

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