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Original ArticleOpen Access
Isomeric Separation of Methamphetamine by HPLC Chiral Column
Lekskulchai V 
Methamphetamine and its active metabolite, amphetamine, are optically active compounds
which, based upon synthetic routes, can be found in two forms; pure d-form and racemic mixture.
Analysis of their isomers can help to identify which precursor is currently spreading widely in a
given region. Since there are many drugs that can be metabolized to amphetamine/methampheta-
mine, isomeric separation can be a useful tool for evaluation of these drugs, as well. Indirect
method by using N-trifluoroacetyl-1 -prolyl chloride
(1 -
TPC) was found to have limited accuracy
due to the contribution effect. In this presentation a direct method using HPLC Chirex chiral
column 3022 was studied. Although the method gave no base-line separation of two different
isomer peaks, it gave good sensitivity, reliability, and linearity. No contribution effect was found
in the method presented. It also gave excellent correlation with the
1-
TPC method.
Key word
: Methamphetamine, Isomeric Separation, Chiral Column
which, based upon synthetic routes, can be found in two forms; pure d-form and racemic mixture.
Analysis of their isomers can help to identify which precursor is currently spreading widely in a
given region. Since there are many drugs that can be metabolized to amphetamine/methampheta-
mine, isomeric separation can be a useful tool for evaluation of these drugs, as well. Indirect
method by using N-trifluoroacetyl-1 -prolyl chloride
(1 -
TPC) was found to have limited accuracy
due to the contribution effect. In this presentation a direct method using HPLC Chirex chiral
column 3022 was studied. Although the method gave no base-line separation of two different
isomer peaks, it gave good sensitivity, reliability, and linearity. No contribution effect was found
in the method presented. It also gave excellent correlation with the
1-
TPC method.
Key word
: Methamphetamine, Isomeric Separation, Chiral Column
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