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Original ArticleOpen Access
Anti-HIV-1 Antibody Testing Using Modified Gelatin Particle Agglutination : A Large Field Study
Louisirirotchanakul S 
,
Kanoksinsombat C ,
Thongput A ,
Puthavathana P ,
Wasi C
,
Kanoksinsombat C ,
Thongput A ,
Puthavathana P ,
Wasi C Anti-HIV testing using gelatin particle agglutination (GPA) assay was investigated in
parallel with ELISAs from routine service at Siriraj Hospital. In the first strategy, 174,032 sera
from a patient population with an HIV-1 seroprevalence of 13.72 per cent were assayed using
reduced volumes of GPA reagents, giving a cost reduction of 40 per cent. In the second strategy,
90,560 pregnant women and 48,936 emigrant workers with an HIV-1 seroprevalence of 2.2 per
cent and 0.3 per cent, respectively, were tested in pools of 4 sera using the manufacturer's recom-
mended volumes, giving a cost saving of 67 per cent. Overall, the sensitivity and specificity were
almost identical with standard methods. Thus, parallel use of either modified GPA might be con-
sidered appropriate when testing large numbers of samples. However, both modified versions of
GPA are not recommended as the first assay for diagnostic or blood bank screening especially in
high prevalence of HIV infection.
Key word : Anti-HIV, Gelatin Particle Assay, Reduced Volume, Pooled Sera
parallel with ELISAs from routine service at Siriraj Hospital. In the first strategy, 174,032 sera
from a patient population with an HIV-1 seroprevalence of 13.72 per cent were assayed using
reduced volumes of GPA reagents, giving a cost reduction of 40 per cent. In the second strategy,
90,560 pregnant women and 48,936 emigrant workers with an HIV-1 seroprevalence of 2.2 per
cent and 0.3 per cent, respectively, were tested in pools of 4 sera using the manufacturer's recom-
mended volumes, giving a cost saving of 67 per cent. Overall, the sensitivity and specificity were
almost identical with standard methods. Thus, parallel use of either modified GPA might be con-
sidered appropriate when testing large numbers of samples. However, both modified versions of
GPA are not recommended as the first assay for diagnostic or blood bank screening especially in
high prevalence of HIV infection.
Key word : Anti-HIV, Gelatin Particle Assay, Reduced Volume, Pooled Sera
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