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Original ArticleOpen Access
The Observation of Immunosuppressor(s) Derived from Cultured Tumor Cells and Its Partial Neutralization with OK-432
Wongkajomsilp A 
,
Luankoso;chai R ,
Huabprasen S ,
Chanyavanich V ,
Tisavipat N ,
Watanapa P
,
Luankoso;chai R ,
Huabprasen S ,
Chanyavanich V ,
Tisavipat N ,
Watanapa P RUNG-ARUNE LUANKOSOLCHAI, M.S.*,
VORAVUT CliANYAVANICH,M.D.**,
PRASIT W ATANAPA, M.D., Ph.D.**
Malignant tumors such as brain tumors have been reported to be associated with
immunosuppression caused by certain tumor-secreted cytokines. The reversion of tumor-derived
immunosuppression has not been described. The use of OK-432, an immunomodulat\>ry
agent prepared from Su-strain of
Streptococcus pyogenes
A3, to activate peripheral blood
mononuclear cells from a patient with glioblastoma multiforme has demonstrated a sharp
rise in proliferative response. This proliferative response was compromised in the presence
of living and irradiated autogeneic cancer cells. The conditioned media from cultured cells
of glioblastoma multiforme, astrocytoma, and cholangiocarcinoma were tested for immuno-
suppressive ability. We found that conditioned media from 3 of 4 cases of glioblastoma,
all 3 cases of astrocytoma, and 1 case of cholangiocarcinoma exhibited immunosuppressive
activity toward the proliferative response of allogeneic peripheral blood mononuclear cells
to phytohemagglutinin. This is the first report that cholangiocarcinoma produces soluble
immunosuppressor(s). Our finding suggested that soluble substance(s) as well as direct
cell-cell contact between tumor cells and mononuclear cells play roles in the observed tumor-
derived immunosuppression.
Key word : Cancer, lmmunosuppressor, Immunomodulator, Cholangiocarcinoma, Brain Tumor,
Glioblastoma Multiforme, Astrocytoma
VORAVUT CliANYAVANICH,M.D.**,
PRASIT W ATANAPA, M.D., Ph.D.**
Malignant tumors such as brain tumors have been reported to be associated with
immunosuppression caused by certain tumor-secreted cytokines. The reversion of tumor-derived
immunosuppression has not been described. The use of OK-432, an immunomodulat\>ry
agent prepared from Su-strain of
Streptococcus pyogenes
A3, to activate peripheral blood
mononuclear cells from a patient with glioblastoma multiforme has demonstrated a sharp
rise in proliferative response. This proliferative response was compromised in the presence
of living and irradiated autogeneic cancer cells. The conditioned media from cultured cells
of glioblastoma multiforme, astrocytoma, and cholangiocarcinoma were tested for immuno-
suppressive ability. We found that conditioned media from 3 of 4 cases of glioblastoma,
all 3 cases of astrocytoma, and 1 case of cholangiocarcinoma exhibited immunosuppressive
activity toward the proliferative response of allogeneic peripheral blood mononuclear cells
to phytohemagglutinin. This is the first report that cholangiocarcinoma produces soluble
immunosuppressor(s). Our finding suggested that soluble substance(s) as well as direct
cell-cell contact between tumor cells and mononuclear cells play roles in the observed tumor-
derived immunosuppression.
Key word : Cancer, lmmunosuppressor, Immunomodulator, Cholangiocarcinoma, Brain Tumor,
Glioblastoma Multiforme, Astrocytoma
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