Views: 1,475 | Downloads:
29
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
Chromosomal Analysis of Failed-fertilized" Human Oocytes Resulting from In-vitro Fertilization and Intracytoplasmic Sperm Injection"
Kunathikom S 
,
Makemaham O ,
Suksompong S ,
Laokirkkiat P
,
Makemaham O ,
Suksompong S ,
Laokirkkiat P ORA WAN MAKEMAHARN, M.Sc. *,
PITAK LAOKIRKKIAT, M.D.*
To evaluate the incidence of chromosomal abnormalities in "failed-fertilized" oocytes
derived from
in-vitro
fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure, a
cytogenetic analysis was performed on 164 IVF and 64 ICSI oocytes. One hundred and eleven
(67.7%) of the IVF and 56 (87.5%) of the ICSI oocytes were successfully karyotyped. Of 111 IVF
oocytes, 73 (65.8%) exhibited normal haploid and 38 (34.2%) were abnormal. The abnormalities
included 25 aneuploid (22.5%) (7 hyperhaploid and 18 hypohaploid), 11 diploid (9.9%) and 2
structural anomalies (1.8%). Of 56 ICSI oocytes, 52 (92.8%) were normal haploid and only 4
(7.2%) were aneuploid, with 2 hyperhaploid and 2 hypohaploid. The sperm nuclei were observed
in 43 IVF oocytes (38.7%), composed of 38 (34.2%) premature chromosome condensation (PCC)
and 5 (4.5%) decondensed sperm heads. Evidence of successful sperm delivery was found in all
56 ICSI oocytes; 25.0 per cent (14/56) showed PCC, 17.9 per cent (10/56) showed decondensed
sperm heads, and 57.1 per cent (32/56) showed intact sperm heads. This study suggested that
about one-third of unfertilized oocytes exhibited chromosomal abnormalities. The difference of
aneuploidy between IVF and ICSI oocytes needs further studies analysing a larger number of
oocytes.
Key word : Chromosomal Analysis, "Failed-fertilized" Oocytes,
In-vitro
Fertilization, Intracyto-
plasmic Sperm Injection
PITAK LAOKIRKKIAT, M.D.*
To evaluate the incidence of chromosomal abnormalities in "failed-fertilized" oocytes
derived from
in-vitro
fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure, a
cytogenetic analysis was performed on 164 IVF and 64 ICSI oocytes. One hundred and eleven
(67.7%) of the IVF and 56 (87.5%) of the ICSI oocytes were successfully karyotyped. Of 111 IVF
oocytes, 73 (65.8%) exhibited normal haploid and 38 (34.2%) were abnormal. The abnormalities
included 25 aneuploid (22.5%) (7 hyperhaploid and 18 hypohaploid), 11 diploid (9.9%) and 2
structural anomalies (1.8%). Of 56 ICSI oocytes, 52 (92.8%) were normal haploid and only 4
(7.2%) were aneuploid, with 2 hyperhaploid and 2 hypohaploid. The sperm nuclei were observed
in 43 IVF oocytes (38.7%), composed of 38 (34.2%) premature chromosome condensation (PCC)
and 5 (4.5%) decondensed sperm heads. Evidence of successful sperm delivery was found in all
56 ICSI oocytes; 25.0 per cent (14/56) showed PCC, 17.9 per cent (10/56) showed decondensed
sperm heads, and 57.1 per cent (32/56) showed intact sperm heads. This study suggested that
about one-third of unfertilized oocytes exhibited chromosomal abnormalities. The difference of
aneuploidy between IVF and ICSI oocytes needs further studies analysing a larger number of
oocytes.
Key word : Chromosomal Analysis, "Failed-fertilized" Oocytes,
In-vitro
Fertilization, Intracyto-
plasmic Sperm Injection
Download:
PDF