J Med Assoc Thai 2001; 84 (6):864

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Evaluation of katG Codon 315 Mutation Among Isoniazid Sensitive and Resistant Mycobacterium tuberculosis Isolates from Thailand
lmwidthaya P Mail, Mieskes K , Rienthong S

Nine isoniazid (INH)-susceptible and 11 INH-resistant
Mycobacterium tuberculosis
clini-
cal isolates were analyzed for
katG
codon 315 mutations by polymerase chain reaction (PCR) assay
using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After
Acil
digestion of PCR products, all 9 INH-susceptible isolates and 5 out of 11 ( 45%) INH-resistant
isolates showed 0.12 kb band, which was previously reported to indicate wild type, whereas, 6 of
11 (55%) INH-resistant isolates lacked this band.
Key word :
M.
tuberculosis, katG
Codon, INH Susceptibility

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