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Original ArticleOpen Access
Pueraria Mirifica, Phytoestrogen-Induced Change in Synaptophysin Expression Via Estrogen Receptor in Rat Hippocampal Neuron
Chindewa R 
,
Lapanantasin S ,
Sanvarinda Y ,
Chongthammakun S
,
Lapanantasin S ,
Sanvarinda Y ,
Chongthammakun S Objective: To examine Pueraria mirifica (Leguminosae) containing-phytoestrogen effect on synaptic density
and involvement of estrogen receptor.
Material and Method: The level of synaptophysin, a presynaptic vesicle protein, was measured using Western
blot analysis and immunocytochemistry in hippocampal primary cell cultures at 6 days in vitro.
Results: P. mirifica and 17β-estradiol (0.1 μM) treatment for 4 days, but not for 2 days, significantly increased
synaptophysin immunoreactivity and level of synaptophysin. P. mirifica up to 60 μg/ml resulted in a dose
related increase in the level of synaptophysin immunoreactivity. The classical estrogen receptor antagonist,
ICI 182 780, significantly blocked P. mirifica-induced increase in synaptophysin.
Conclusion: P. mirifica-containing phytoestrogen affects synaptic density by inducing synaptophysin expression
via estrogen receptor.
Keywords: Phytoestrogens, Pueraria mirifica, Hippocampal neuron, 17β-estradiol, Synaptophysin, Synaptic
density
and involvement of estrogen receptor.
Material and Method: The level of synaptophysin, a presynaptic vesicle protein, was measured using Western
blot analysis and immunocytochemistry in hippocampal primary cell cultures at 6 days in vitro.
Results: P. mirifica and 17β-estradiol (0.1 μM) treatment for 4 days, but not for 2 days, significantly increased
synaptophysin immunoreactivity and level of synaptophysin. P. mirifica up to 60 μg/ml resulted in a dose
related increase in the level of synaptophysin immunoreactivity. The classical estrogen receptor antagonist,
ICI 182 780, significantly blocked P. mirifica-induced increase in synaptophysin.
Conclusion: P. mirifica-containing phytoestrogen affects synaptic density by inducing synaptophysin expression
via estrogen receptor.
Keywords: Phytoestrogens, Pueraria mirifica, Hippocampal neuron, 17β-estradiol, Synaptophysin, Synaptic
density
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