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Original ArticleOpen Access
Comparison of Embryonic Development in Cleavage Stage Mouse Embryo Biopsy Between Acid Tyrode's Solution and Laser Assisted Techniques
Phophong P 
,
Doshi A ,
Harper JC
,
Doshi A ,
Harper JC PHAKPHUM PHOPHONG, M.D., M.Sc.*,
ALPESH DOSHI, M.Sc.
**,
JOYCE C HARPER, Ph.D.***
The study was carried out to detennine the effectiveness and safety of the infrared 1.48
~m
laser in cleavage stage mouse embryo biopsy, compared to the conventional acid Tyrode's
solution. One hundred and thirty cryopreserved cleavage stage mouse embryos were included
in the study. Fifty embryos were biopsied by acid Tyrode's solution. Forty-seven embryos were
biopsied by the infrared 1.48
~m
laser. Thirty-three embryos were incubated without biopsy as
the control group. Thirteen of 50 embryos in the acid Tyrode's group and 16 of 47 in the laser
assisted group became cavitating morulae on day 4, meanwhile 23 of 33 in the control group
reached this stage. The blastocyst fonnation of acid Tyrode's, laser assisted and control group
were 94.0, 97.8 and 100.0 per cent, respectively. The hatching rate of acid Tyrode's solution, laser
assisted and control group were 78.7, 84.7 and 63.6 per cent, respectively. No significant diffe-
rence in blastocyst fonnation and hatching rate was found. The percentage of grade
I
blastocysts
in control group (96.9%) was significantly higher than those in acid Tyrode's solution (68.0%)
and the laser assisted group (76.0% ). There was no significant difference in the percentage of
grade 1 blastocysts between the acid Tyrode's solution and the laser assisted group. In conclusion,
the infrared 1.48
~m
wavelength laser may be an alternative to acid Tyrode's solution in embryo
biopsy.
Key word
Laser, Acid Tyrode's Solution, Embryo Biopsy, Preimplantation Genetic Diagnosis,
Blastocyst
ALPESH DOSHI, M.Sc.
**,
JOYCE C HARPER, Ph.D.***
The study was carried out to detennine the effectiveness and safety of the infrared 1.48
~m
laser in cleavage stage mouse embryo biopsy, compared to the conventional acid Tyrode's
solution. One hundred and thirty cryopreserved cleavage stage mouse embryos were included
in the study. Fifty embryos were biopsied by acid Tyrode's solution. Forty-seven embryos were
biopsied by the infrared 1.48
~m
laser. Thirty-three embryos were incubated without biopsy as
the control group. Thirteen of 50 embryos in the acid Tyrode's group and 16 of 47 in the laser
assisted group became cavitating morulae on day 4, meanwhile 23 of 33 in the control group
reached this stage. The blastocyst fonnation of acid Tyrode's, laser assisted and control group
were 94.0, 97.8 and 100.0 per cent, respectively. The hatching rate of acid Tyrode's solution, laser
assisted and control group were 78.7, 84.7 and 63.6 per cent, respectively. No significant diffe-
rence in blastocyst fonnation and hatching rate was found. The percentage of grade
I
blastocysts
in control group (96.9%) was significantly higher than those in acid Tyrode's solution (68.0%)
and the laser assisted group (76.0% ). There was no significant difference in the percentage of
grade 1 blastocysts between the acid Tyrode's solution and the laser assisted group. In conclusion,
the infrared 1.48
~m
wavelength laser may be an alternative to acid Tyrode's solution in embryo
biopsy.
Key word
Laser, Acid Tyrode's Solution, Embryo Biopsy, Preimplantation Genetic Diagnosis,
Blastocyst
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