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Original ArticleOpen Access
In Vitro Susceptibility Testing of Levofloxacin and Ofloxacin by Microliter Plate Alamar Blue Against Multidrug and Non Multidrug Resistant Mycobacterium tuberculosis in Thailand
Pracharktam R 
,
Angkananukool K ,
Vibhagool A
,
Angkananukool K ,
Vibhagool A Antituberculous drugs for current therapy of multidrugs resistant tuberculosis (MDR-TB)
are limited. The
in vitro
susceptibility of
Mycobacterium tuberculosis
(MTB) as well as MDR-
TB with other antibiotic drugs were determined in order to find alternative drugs for the treat-
ment of MDR-TB. Forty-seven MDR-TB and 62 MTB of clinical isolates were tested against
levofloxacin and ofloxacin. The MDR-TB at the MIC
90
of levofloxacin and ofloxcain were
1
J.lg/
ml and 2 J.lg/ml, respectively. Of these MTB, the MIC
90
of both drugs were 0.5 f.lg/ml and 1
f.lg/ml, respectively. It seemed that levofloxacin MICs of both MDR-TB and MTB were one
dilution less than ofloxcin. The promising activity of ofloxacin and levofloxacin against MDR-
TB and MTB suggest that both drugs could be used as second-line drugs for MDR-TB or as a
good alternative antituberculous drug for patients who have intolerance to the first line drug.
Key word :
Mycobacterium tuberculosis,
Tuberculosis, Levofloxacin, Ofloxacin, Alamar Blue
are limited. The
in vitro
susceptibility of
Mycobacterium tuberculosis
(MTB) as well as MDR-
TB with other antibiotic drugs were determined in order to find alternative drugs for the treat-
ment of MDR-TB. Forty-seven MDR-TB and 62 MTB of clinical isolates were tested against
levofloxacin and ofloxacin. The MDR-TB at the MIC
90
of levofloxacin and ofloxcain were
1
J.lg/
ml and 2 J.lg/ml, respectively. Of these MTB, the MIC
90
of both drugs were 0.5 f.lg/ml and 1
f.lg/ml, respectively. It seemed that levofloxacin MICs of both MDR-TB and MTB were one
dilution less than ofloxcin. The promising activity of ofloxacin and levofloxacin against MDR-
TB and MTB suggest that both drugs could be used as second-line drugs for MDR-TB or as a
good alternative antituberculous drug for patients who have intolerance to the first line drug.
Key word :
Mycobacterium tuberculosis,
Tuberculosis, Levofloxacin, Ofloxacin, Alamar Blue
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