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Original ArticleOpen Access
Endemic Bancroftian Filariasis in Thailand: Detection by Og4C3 Antigen Capture ELISA and the Polymerase Chain Reaction
Nuchprayoon S 
,
Yentakam S ,
Sangprakarn S ,
Junpee A
,
Yentakam S ,
Sangprakarn S ,
Junpee A Lymphatic filariasis, mainly caused
by Wuchereria bancrofti
and
Brugia malayi,
has been
targeted for elimination by the World Health Organization by the year 2020. To achieve this goal,
highly sensitive and specific diagnostic tests are necessary for close monitoring and evaluation of
the control program. We employed an ELISA to detect the Og4C3 antigen and a polymerase chain
reaction-based assay for diagnosis of
W. bancrofti
infection, among the Thai-Karen population in Tak
province, Thailand. We found that this endemic area had a microfilarial rate of 10 per cent, while
the antigen assay could detect cases about two fold as many (23% ). The repeated PCR for the detec-
tion of
Ssp
I of
W. bancrofti
was positive in 12 per cent of the population under this study. Our data
emphasize the need for using highly sensitive and specific assays for assessment of the real burden of
the disease.
Key word :
Wuchereria bancrofti, Ssp
I repeat, Og4C3 Antigen, PCR, Antigen Detection Assay
by Wuchereria bancrofti
and
Brugia malayi,
has been
targeted for elimination by the World Health Organization by the year 2020. To achieve this goal,
highly sensitive and specific diagnostic tests are necessary for close monitoring and evaluation of
the control program. We employed an ELISA to detect the Og4C3 antigen and a polymerase chain
reaction-based assay for diagnosis of
W. bancrofti
infection, among the Thai-Karen population in Tak
province, Thailand. We found that this endemic area had a microfilarial rate of 10 per cent, while
the antigen assay could detect cases about two fold as many (23% ). The repeated PCR for the detec-
tion of
Ssp
I of
W. bancrofti
was positive in 12 per cent of the population under this study. Our data
emphasize the need for using highly sensitive and specific assays for assessment of the real burden of
the disease.
Key word :
Wuchereria bancrofti, Ssp
I repeat, Og4C3 Antigen, PCR, Antigen Detection Assay
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