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Original ArticleOpen Access
The Analysis of Peri-Tumor Necrosis Following the Subcutaneous Implantation of Autologous Tumor Cells Transfected with an Episome Transcribing an Antisense Insulin-Like Growth Factor 1 RNA in a Glioblastoma Multiforme Subject
Wongkajomsilp A 
,
Tisavipat N ,
Ouyprasertkul M ,
Chanyavanich V ,
Sangruchi T ,
Sangruchi S ,
Huabprasert S ,
Chomsamut K ,
Pan Y ,
Anthony DD
,
Tisavipat N ,
Ouyprasertkul M ,
Chanyavanich V ,
Sangruchi T ,
Sangruchi S ,
Huabprasert S ,
Chomsamut K ,
Pan Y ,
Anthony DD NANTASAK TISAVIPAT, M.D.**,
VORA VUT CHANYAVANICH, M.D.**,
SUPATRA SANGRUCHI, M.D.****,
KARUNA CHOMSAMUT, B.Sc.**,
DONALD D. ANTHONY, M.D.*****
A subject inflicted with glioblastoma multiforme who received partial tumor resection and
radiotherapy was recruited for an
ex vivo
gene therapy protocol using irradiated autologous tumor
cells that had been engineered to suppress the expression of insulin-like growth factor I as the
tumor vaccine. After subcutaneous injection for 8 weeks, the subject developed peri-tumor necro-
sis with mass effect. The authors wondered whether this event could have resulted from the tumor
vaccine. The tissue section bordering the necrotic tumor tissue to the viable normal tissue was
examined for nature of any infiltrated cells and their activities. Lymphocytes, macrophages, and a
small number of neutrophils diffused into the necrotic tumor tissue were found. The infiltrated
lymphocytes consisted of both CD4+ and CDS+ T cells. The functional activity of these lympho-
cytes was demonstrated by the active production of interferon
y
and tumor necrosis factor a based
on the respective immunofluorescent staining localized to these cells. This finding is compatible
with the proposed mechanism underlying the tumor vaccination. However, the contribution of radia-
tion treatment to this event cannot be clearly ruled out.
Key word :Peri-Tumor Necrosis, Insulin-Like Growth Factor 1, Glioblastoma Multiforme, Gene
Therapy
VORA VUT CHANYAVANICH, M.D.**,
SUPATRA SANGRUCHI, M.D.****,
KARUNA CHOMSAMUT, B.Sc.**,
DONALD D. ANTHONY, M.D.*****
A subject inflicted with glioblastoma multiforme who received partial tumor resection and
radiotherapy was recruited for an
ex vivo
gene therapy protocol using irradiated autologous tumor
cells that had been engineered to suppress the expression of insulin-like growth factor I as the
tumor vaccine. After subcutaneous injection for 8 weeks, the subject developed peri-tumor necro-
sis with mass effect. The authors wondered whether this event could have resulted from the tumor
vaccine. The tissue section bordering the necrotic tumor tissue to the viable normal tissue was
examined for nature of any infiltrated cells and their activities. Lymphocytes, macrophages, and a
small number of neutrophils diffused into the necrotic tumor tissue were found. The infiltrated
lymphocytes consisted of both CD4+ and CDS+ T cells. The functional activity of these lympho-
cytes was demonstrated by the active production of interferon
y
and tumor necrosis factor a based
on the respective immunofluorescent staining localized to these cells. This finding is compatible
with the proposed mechanism underlying the tumor vaccination. However, the contribution of radia-
tion treatment to this event cannot be clearly ruled out.
Key word :Peri-Tumor Necrosis, Insulin-Like Growth Factor 1, Glioblastoma Multiforme, Gene
Therapy
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