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Original ArticleOpen Access
Antibodies to Leptospirosis in Rodents from Thailand Using a Modified Human Diagnostic Assay
M. T 
,
Phulsuksombati D ,
Kingnate D ,
Prachumsri J ,
Rachphaew N ,
Monkanna T ,
Gettayakamin M
,
Phulsuksombati D ,
Kingnate D ,
Prachumsri J ,
Rachphaew N ,
Monkanna T ,
Gettayakamin M The number of reported cases of Leptospirosis in Thailand has grown since 1996. Identifi-
cation of major reservoirs and endemic areas is essential in surveillance of
Leptospira
species in
Thailand. To assist in the effort of surveillance, a dipstick assay for detecting
Leptospira
antibodies
in mammals was adapted from a human diagnostic assay and tested in a field trial in Thailand. Anti-
bodies to
Leptospira
were detected in 18 of 60 wild rodents. Four of 9 culture positive rodents
were positive by the dipstick assay. The proportion of sera positive for antibodies by dipstick was
correlated with positive culture outcome using McNemar test for correlated proportions (0.83, P>
0.05). The dipstick assay was effective in detecting antibodies to
Leptospira
in mammals and may
be useful in resource poor areas or under circumstances where the microagglutination test (MAT)
is not practical.
Key word : Leptospirosis, Rodent, Rattus, Antibody, Mammal
cation of major reservoirs and endemic areas is essential in surveillance of
Leptospira
species in
Thailand. To assist in the effort of surveillance, a dipstick assay for detecting
Leptospira
antibodies
in mammals was adapted from a human diagnostic assay and tested in a field trial in Thailand. Anti-
bodies to
Leptospira
were detected in 18 of 60 wild rodents. Four of 9 culture positive rodents
were positive by the dipstick assay. The proportion of sera positive for antibodies by dipstick was
correlated with positive culture outcome using McNemar test for correlated proportions (0.83, P>
0.05). The dipstick assay was effective in detecting antibodies to
Leptospira
in mammals and may
be useful in resource poor areas or under circumstances where the microagglutination test (MAT)
is not practical.
Key word : Leptospirosis, Rodent, Rattus, Antibody, Mammal
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