Views: 1,369 | Downloads:
25
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
p53 Tumor Suppressor Gene Mutation in Ovarian Cancer in Thai Patients
Neungton N 
,
Neungton S ,
Leelaphatanadit C ,
Dangrat C ,
Soiampornkul R
,
Neungton S ,
Leelaphatanadit C ,
Dangrat C ,
Soiampornkul R SOMCHA YA NEUNGTON, M.D.**,
CHONGDEE DANGRAT, M.Sc.**,
Objectives
: To characterize molecular mutations of
p53
gene in Thai ovarian cancer and
compare the mutations with their pathological and clinical findings.
Material and Method
: Direct DNA sequencing of hot spot region of
p53
gene (exons 5
to 8) from 28 primary ovarian cancer tissues, 2 metastatic tumors and their paired blood samples
was performed. The detected mutations were compared to the pathological and clinical findings and
responsiveness to treatments after 36 months of follow-up.
Results
: One insertion and 4 point mutations in exon 5 of
p53
gene were found in 5 out
of 28 (18%) ovarian cancer patients. There was no mutation in the paired blood samples. The histo-
logical types of the detected tumors were 3 endometrioids and 2 serous cystadenocarcinomas. All
5 patients were in stage I to IV disease and showed overall 4 out of 5 (80%) complete response
until 36 months after surgery followed by chemotherapy, compared to 14 out of 28 (50%) of complete
response in all cases of ovarian cancer.
Conclusion
: The authors found 5 cases of ovarian cancer patients with
p53
gene mutations
giving the same response to complete standard treatment as all cases. Significant factors affecting
responsiveness of these patients depended more on stages, grades and histological cell types of the
cancer.
Key word
: Ovarian Neoplasm, Gene
p53
Point Mutation, Molecular Sequence Data, Prognosis
CHONGDEE DANGRAT, M.Sc.**,
Objectives
: To characterize molecular mutations of
p53
gene in Thai ovarian cancer and
compare the mutations with their pathological and clinical findings.
Material and Method
: Direct DNA sequencing of hot spot region of
p53
gene (exons 5
to 8) from 28 primary ovarian cancer tissues, 2 metastatic tumors and their paired blood samples
was performed. The detected mutations were compared to the pathological and clinical findings and
responsiveness to treatments after 36 months of follow-up.
Results
: One insertion and 4 point mutations in exon 5 of
p53
gene were found in 5 out
of 28 (18%) ovarian cancer patients. There was no mutation in the paired blood samples. The histo-
logical types of the detected tumors were 3 endometrioids and 2 serous cystadenocarcinomas. All
5 patients were in stage I to IV disease and showed overall 4 out of 5 (80%) complete response
until 36 months after surgery followed by chemotherapy, compared to 14 out of 28 (50%) of complete
response in all cases of ovarian cancer.
Conclusion
: The authors found 5 cases of ovarian cancer patients with
p53
gene mutations
giving the same response to complete standard treatment as all cases. Significant factors affecting
responsiveness of these patients depended more on stages, grades and histological cell types of the
cancer.
Key word
: Ovarian Neoplasm, Gene
p53
Point Mutation, Molecular Sequence Data, Prognosis
Download:
PDF