J Med Assoc Thai 2003; 86 (11):1055

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Collection and Processing of Umbilical Cord Blood for Cryopreservation Yaowalak U-pratya, MS, Sirikwan Boonmoh, BSc, Orathai Promsuwicha, BSc, Chanya Theerapitayanon, MS, Laksami Kalanchai, BSc, Viruch Chanjerboon, MS,
Sirimai K Mail, Visuthisakchai S , Bejrachandra S , S

SIRIKW AN BOONMOH, BSc*,
CHANYA THEERAPITAYANON, MS*,
VIRUCH CHANJERBOON, MS**,
SANAN VISUTHISAKCHAI, MD*,
SURAPOL ISSARAGRISIL, MD*
Umbilical cord blood (UCB) is being increasingly used as an alternative source of hemato-
poietic stem cells for allogeneic bone marrow transplantation. UCB transplantation has been success-
fully used to treat a variety of genetic, hematological, and oncological disorders in children and adults.
The objectives of this study was to establish a closed-system technique for UCB collection and buffy
coat separation by Optipress I device. Thirty-four UCB were collected by triple-bag system from preg-
nant mothers whose fetuses were not affected by thalassemic diseases after prenatal diagnosis. The
mean volumn of UCB collection were 120เธ‘5 ml (range 65-180 ml). Total WBC, CD34+ cells, the
progenitor cell erythroid burst-forming unit (BFU-E) and granulocyte-macrophage colony-forming unit
(CFU-GM) in the UCB units were (9.36 เธ‘ 0.84) x 108
,
(3.61 เธ‘ 0.52) x 106
,
(9.12 เธ‘ 1.60) x 105
,
and
(5.32 เธ‘ 1.23) x 105
,
respectively. Good correlation between the nucleated cell and net cord blood volume
could be demonstrated (p
<
0.0001). The correlation between CD34+ cells and the following para-
meters: nucleated cell, BFU-E or CFU-GM were also demonstrated (p
=
0.001, 0.0105 or 0.0001, res-
pectively). Buffy coat was subsequently separated from 18 UCB units by Optipress I device. 70 เธ‘ 3 ml
of buffy coat were collected and cryoprocessing was done by automatic controlled-rate freezer. Good
recovery .of total WBC, CD34+ cells, progenitor cells BFU-E and CFU-GM after buffy coat separa-
tion were observed 89 per cent, 95 per cent, 109 per cent, and 102 per cent respectively. There was no
1056
Y. U-PRATYA et

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