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Original ArticleOpen Access
Mouse Blastocyst Vitrification Compared with the Conventional Slow- Freezing Method
MD WT 
,
Pruksananonda K ,
Boonyakasemsanti W ,
Virutamasen P ,
Ahnonkitpanit V ,
Chompurat D ,
Numchaisrika P
,
Pruksananonda K ,
Boonyakasemsanti W ,
Virutamasen P ,
Ahnonkitpanit V ,
Chompurat D ,
Numchaisrika P SOMCHAI SUWAJANAKORN, MD*,
KUMTHORN PRUKSANANONDA, MD*,
PRAMUAN VIRUTAMASEN, MD*,
DOENTIP CHOMPURAT, BSc*,
To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-
freezing method, one-hundred and forty-eight
in vitro
mouse blastocysts were randomly frozen by the
two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were
assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrifica-
tion and slow-freezing were 68.33 and 65.52 per cent (p
=
0.89), whereas hatching rates were 51.22 and
44.74 per cent, respectively (p
=
0.64).Therefore, vitrification of blastocyst-stage-embryos may be a
useful, economic method for freezing the excess blastocysts in some centers where blastocysts are
routinely transferred.
Key word
: Mouse Blastocyst, Cryopreservation, Vitrification, Conventional, Slow-Freezing Method
KUMTHORN PRUKSANANONDA, MD*,
PRAMUAN VIRUTAMASEN, MD*,
DOENTIP CHOMPURAT, BSc*,
To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-
freezing method, one-hundred and forty-eight
in vitro
mouse blastocysts were randomly frozen by the
two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were
assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrifica-
tion and slow-freezing were 68.33 and 65.52 per cent (p
=
0.89), whereas hatching rates were 51.22 and
44.74 per cent, respectively (p
=
0.64).Therefore, vitrification of blastocyst-stage-embryos may be a
useful, economic method for freezing the excess blastocysts in some centers where blastocysts are
routinely transferred.
Key word
: Mouse Blastocyst, Cryopreservation, Vitrification, Conventional, Slow-Freezing Method
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