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Original ArticleOpen Access
Distribution of Human Leukocyte Antigens-E Alleles in Thailand
Kimkong l 
,
Mutirangura A ,
Pimtanothai N
,
Mutirangura A ,
Pimtanothai N The aim of this study was to investigate the distribution of human leukocyte antigens (HLA)
-E alleles in Thailand. HLA-E alleles were assigned by using a polymerlase chain reaction-sequence
specific oligonucleotide probes (PCR-SSOP) method and direct sequencing in 200 healthy individuals.
They comprised 100 Thai, 50 Chinese and 50 Thai-Chinese. From the results, three alleles of HLA-E
could be detected in these populations. The E*OlOl was the most common allele in Thai and Thai-
Chinese with allelic frequencies of 42.5 per cent and 38 per cent, respectively. The other HLA-E allele
frequencies of Thai origin were 33 per cent for E*Ol031 and 24.5 per cent for E*Ol032, respectively.
Among Thai-Chinese, the allele frequencies of HLA-E were 31 per cent for E*Ol031 and E*Ol032,
respectively. Whereas, the E*Ol031 was the predominant allele in Chinese origin with a frequency of
39 per cent, followed by E*OlOl and E*Ol032 with 32 per cent and 29 per cent, respectively. No
E*Ol033, E*Ol02 and E*Ol04 could be detected in all individuals. When comparing the distribution of
HLA-E alleles between each of the populations (Thai
vs
Chinese, Thai
vs
Thai-Chinese and Chinese
vs
Thai-Chinese), no significant difference could be found among these populations. In addition, there
was no significant difference of the distribution of HLA-E alleles between the study populations and
other populations from Asian countries, reported previously. However, there were significant diffe-
rences between the populations (Thai, Chinese and Thai-Chinese) and Danish (X2
=
15.64, p
=
0.0004;
X2
=
24.58, p
=
0.0000046; X2
=
14.69, p
=
0.00065, respectively).
Key word : HLA-E, Alleles, PCR-SSOP, Direct Sequencing
-E alleles in Thailand. HLA-E alleles were assigned by using a polymerlase chain reaction-sequence
specific oligonucleotide probes (PCR-SSOP) method and direct sequencing in 200 healthy individuals.
They comprised 100 Thai, 50 Chinese and 50 Thai-Chinese. From the results, three alleles of HLA-E
could be detected in these populations. The E*OlOl was the most common allele in Thai and Thai-
Chinese with allelic frequencies of 42.5 per cent and 38 per cent, respectively. The other HLA-E allele
frequencies of Thai origin were 33 per cent for E*Ol031 and 24.5 per cent for E*Ol032, respectively.
Among Thai-Chinese, the allele frequencies of HLA-E were 31 per cent for E*Ol031 and E*Ol032,
respectively. Whereas, the E*Ol031 was the predominant allele in Chinese origin with a frequency of
39 per cent, followed by E*OlOl and E*Ol032 with 32 per cent and 29 per cent, respectively. No
E*Ol033, E*Ol02 and E*Ol04 could be detected in all individuals. When comparing the distribution of
HLA-E alleles between each of the populations (Thai
vs
Chinese, Thai
vs
Thai-Chinese and Chinese
vs
Thai-Chinese), no significant difference could be found among these populations. In addition, there
was no significant difference of the distribution of HLA-E alleles between the study populations and
other populations from Asian countries, reported previously. However, there were significant diffe-
rences between the populations (Thai, Chinese and Thai-Chinese) and Danish (X2
=
15.64, p
=
0.0004;
X2
=
24.58, p
=
0.0000046; X2
=
14.69, p
=
0.00065, respectively).
Key word : HLA-E, Alleles, PCR-SSOP, Direct Sequencing
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