J Med Assoc Thai 2003; 86 (6):299

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Antispasmodic Effects of Curcuminoids on Isolated Guinea-Pig Ileum and Rat Uterus
ltthipanichpong C Mail, Ruangrungsi N , Kemsri W , Sawasdipanich A

NU8IRI RUANGRUNG81, PhD**,
ANUGOOL 8AWA8DIPANICH,M8c*
Curcuminoids, a yellow constituent isolated from
Curcuma longa
Linn. rhizomes was studied
for its antispasmodic activity in isolated guinea-pig ileum and rat uterus. Curcuminoids at the con-
centration of 12 IJ.g/ml significantly inhibited the ileum pre-contracted with acetylcholine (ACh) 5 x I0-7
M .and histamine 5 x I0-
7
M. (Force of contraction was 62.84
เธ‘
4.66% and 75.60 เธ‘ 4.66% respectively)
and the effects were prominently observed when the concentration of curcuminoids was increased to
36 IJ.g/ml. (Force of contraction
~as
44;93
เธ‘
4.33% and 42.79
เธ‘
1.98%) In potassium depolarizing
Tyrode solution, เธ—curcuminoids 41J.g/ml and 20 IJ.g/rtll reduced the contraction induced by calcium chlo-
ride (CaCI
2
)
1.8 mM. (The contraction was 63.31
เธ‘
1.80% and 36.87
เธ‘
3.25%) In rat uterus smooth
muscle preparation, curcuminoids 8 IJ.g/ml and 16 IJ.g/ml significantly reduced force and frequency of
contraction induced by oxytocin I x I0-
2
IU/ml. Curcuminoids 8 IJ.g/ml produced 54.68
เธ‘
3.34 per cent
force of contraction and 79.09
เธ‘
2.29 per cent frequency of contraction. Curcuminoids 161J.g/ml caused
more relaxation of rat uterus smooth muscle. (Force of contraction. was 43.38 เธ‘ 3.56%, frequency of
contraction was 49.96
เธ‘
5.20%). Curcuminoids 8 and 161J.g/ml significantly reduced force of contrac-
tion induced by KCI 50 mM. (Force of contraction was 54.10
เธ‘
4.92% and 36.60
เธ‘
2.99%). The results
obtained from this study concluded that curcuminoids produced a smooth muscle.
re~axation
effect on
isolated guinea-pig ileum and rat uterus by receptor-dependent and independent mechanism:
Key word : Curcuminoids, Antispasmodic, Turmeric

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