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Original ArticleOpen Access
Cord Blood Collection for the National Cord Blood Bank in Thailand
Wacharaprechanont T 
,
0Charoen R ,
Vanichsetakul P ,
Sudjai D ,
Kupatawintu P ,
Seksam P ,
Samritpradit P ,
Charoenvidhya D
,
0Charoen R ,
Vanichsetakul P ,
Sudjai D ,
Kupatawintu P ,
Seksam P ,
Samritpradit P ,
Charoenvidhya D RACHANEE 0-CHAROEN, MD**,
DENNOPPORN SUDJAI, MD*,
PANYA SEKSARN, MD***,
DHIRAPHONGS CHAROENVIDHYA, MD*
Umbilical cord blood is an effective alternative source of hematopoietic stem cells trans-
plantation in children and adolescents. However, the efficacy and safety of cord blood transplantation
correlates with the quantity and quality of cord blood. To evaluate the collection systems and process-
ing of cord blood donations, a pilot research program to optimize recruitment, collection and process-
ing of cord blood donations was developed. The present results showed that the quality of the cord
blood (volume, total white blood cells (WBC) count, CD34+ and sterility control) collected was satis-
factory and discard rate of collecting units (24.2%) were comparable with data reported from other cord
blood banks. To find the optimal mode of collection, comparison of 3 cord blood collection methods
(Method 1
=
Hanging method after delivering the placenta, Method 2
=
Aspiration from
in utero
placenta, Method 3
=
Aspiration from
in utero
placenta and Syringe-assisted aspiration) using the closed
system showed that method 3 was the best method but it required more trained personnel and
involved a complicated procedure. The National Cord Blood Bank started its activity in 2002 after
several years of pre-clinical studies. To date, a number of transplants using cord blood from related and
unrelated cord blood (first report in Thailand) donors have been successfully performed.
Key word
: Umbilical Cord Blood, Collection Methods, Cord Blood Bank, Hematopoietic Stem Cell,
Transplantation
DENNOPPORN SUDJAI, MD*,
PANYA SEKSARN, MD***,
DHIRAPHONGS CHAROENVIDHYA, MD*
Umbilical cord blood is an effective alternative source of hematopoietic stem cells trans-
plantation in children and adolescents. However, the efficacy and safety of cord blood transplantation
correlates with the quantity and quality of cord blood. To evaluate the collection systems and process-
ing of cord blood donations, a pilot research program to optimize recruitment, collection and process-
ing of cord blood donations was developed. The present results showed that the quality of the cord
blood (volume, total white blood cells (WBC) count, CD34+ and sterility control) collected was satis-
factory and discard rate of collecting units (24.2%) were comparable with data reported from other cord
blood banks. To find the optimal mode of collection, comparison of 3 cord blood collection methods
(Method 1
=
Hanging method after delivering the placenta, Method 2
=
Aspiration from
in utero
placenta, Method 3
=
Aspiration from
in utero
placenta and Syringe-assisted aspiration) using the closed
system showed that method 3 was the best method but it required more trained personnel and
involved a complicated procedure. The National Cord Blood Bank started its activity in 2002 after
several years of pre-clinical studies. To date, a number of transplants using cord blood from related and
unrelated cord blood (first report in Thailand) donors have been successfully performed.
Key word
: Umbilical Cord Blood, Collection Methods, Cord Blood Bank, Hematopoietic Stem Cell,
Transplantation
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