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Original ArticleOpen Access
Red Blood Cell Vesicles in Thalassemia
Lamchiagdhase P 
,
Nitipongwanich R ,
Rattanapong C ,
Noulsri E ,
Lerdwana S ,
Pattanapanyasat K
,
Nitipongwanich R ,
Rattanapong C ,
Noulsri E ,
Lerdwana S ,
Pattanapanyasat K RUMANEEYA NITIPONGWANICH,BSc*,
EGARIT NOULSRI, MSc**,
KOVIT PATTANAPANYASAT, PhD**
Vesicles are part of the red blood cells membrane which can be found in a small number in
normal apoptotic process and increased in some diseases. In the present study, the authors measured
the percentage of red blood cell vesicles in healthy subjects (n
=
7), patients with a-thalassemia or
Hemoglobin (Hb) H disease (n
=
7),
13-thal/Hb
E
with nonsplenectomized (n
=
5) and splenectomized
(n
=
7) before and after induction heated at 48.6"C by using flow cytometry. It was found that the
percentage of vesicles in every group were not statistically significantly different (p
>
0.05) between
pre and post incubation at 5 min. The percentage of vesicles of healthy subjects, 13-thal/Hb E non-
splenectomized patients and splenectomized patients were highest when induced by heating for 60 min.
For patients with Hb H disease, the percentage of vesicles was maximum at 30 min when compared
with healthy subjects, 13-thal/Hb
E
nonsplenectomized patients and splenectomized patients, respec-
tively. In the present study, the authors report the significant increase of the percentage of vesicles in
Hb H disease, 13-thal/Hb E nonsplenectomized and splenectomized after induction by heat when com-
pared with healthy subjects. These findings may support the different pathology of the red blood cells
found in a-and 13-thalassemia.
Key word : Vesicles, Thalassemia, Flow Cytometry
EGARIT NOULSRI, MSc**,
KOVIT PATTANAPANYASAT, PhD**
Vesicles are part of the red blood cells membrane which can be found in a small number in
normal apoptotic process and increased in some diseases. In the present study, the authors measured
the percentage of red blood cell vesicles in healthy subjects (n
=
7), patients with a-thalassemia or
Hemoglobin (Hb) H disease (n
=
7),
13-thal/Hb
E
with nonsplenectomized (n
=
5) and splenectomized
(n
=
7) before and after induction heated at 48.6"C by using flow cytometry. It was found that the
percentage of vesicles in every group were not statistically significantly different (p
>
0.05) between
pre and post incubation at 5 min. The percentage of vesicles of healthy subjects, 13-thal/Hb E non-
splenectomized patients and splenectomized patients were highest when induced by heating for 60 min.
For patients with Hb H disease, the percentage of vesicles was maximum at 30 min when compared
with healthy subjects, 13-thal/Hb
E
nonsplenectomized patients and splenectomized patients, respec-
tively. In the present study, the authors report the significant increase of the percentage of vesicles in
Hb H disease, 13-thal/Hb E nonsplenectomized and splenectomized after induction by heat when com-
pared with healthy subjects. These findings may support the different pathology of the red blood cells
found in a-and 13-thalassemia.
Key word : Vesicles, Thalassemia, Flow Cytometry
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