J Med Assoc Thai 2008; 91 (5):608

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Effect of Leukemia Inhibitory Factor (LIF) on the Quality of In Vitro Produced Mouse Blastocysts and Subsequent Derivation of Embryonic Stem (ES) Cells
Rungsiwiwut R Mail, Rungarunlert S , Numchaisrika P , Pruksananonda K , Techakumphu M , Virutamasen P

Objective: To determine the effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse
blastocyst and the efficiency of embryonic stem (ES) cell derivation.

Design: Experimental study

Setting: Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine,
Chulalongkorn University

Material and Method:
In vivo fertilized zygotes were collected and subjected to in vitro culture in potassium
simplex optimized medium (KSOM) containing 1,000 unit/ml LIF. The developmental ability of the zygote to
blastocyst-stage and the cell numbers in blastocysts were evaluated. Expanded blastocysts developed in
different culture media were subsequently subjected to ES cell derivation.

Main Outcome Measure (s): The influence of LIF on the quality of and the total cell numbers of blastocyst
developed in vitro.

Results: Supplementation of LIF in KSOM increased the rate of hatching blastocysts (63.8% vs. 53.7%; p <
0.05) and total cell numbers (91.4 + 15.0 vs. 85.1 + 7.7; p < 0.05) compared to KSOM alone. ES cells were
obtained 66.7% from blastocysts developed in KSOM-LIF versus 41.7% in KSOM (p > 0.05). Established ES
cell lines showed typical colony and characteristics of pluripotent murine ES cells.

Conclusion: LIF improved the quality of in vitro produced blastocysts but not enhanced ES cell derivation.

Keywords: LIF, in vitro, Blastocyst, Embryonic stem cells

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