Background: Prabchompoothaweep remedy (PT) has long been used in Thai traditional medicine to treat allergic rhinitis and asthma. It is composed of 23 plants. It is on National herbal drug list of Thailand, but there is no report for anti-allergic, anti-inflammatory and antioxidant activities.
Objective: To investigate anti-allergic, anti-inflammatory and antioxidant activities of the crude extract from PT by different extraction method.
Material and Method: The method of extract used was maceration in 95% ethanol and 50% ethanol; the residue of these extracts were continued extracted by boiling water, they obtained PTE95, PTE50, PTR95 and PTR50, respectively. The other method of extraction was boiling and drying by lyophilizer that obtained PTW. Five crude extracts were determined antiallergic activity by the inhibition of β-hexosaminidase release from RBL-2H3 cell lines, anti-inflammatory activity were determined by the inhibition of nitric oxide (NO) production from RAW 264.7 cell lines induced by lipopolysaccharide (LPS) and antioxidant activity were tested by DPPH radical scavenging assay.
Results: PTE95 showed the most potent of anti-allergic activity, anti-inflammatory activity and antioxidant activity (IC50 = 12.97, 22.51 and EC50 = 14.62 μg/ml, respectively).
Conclusion: These results suggest that the method of extraction PT that showed the best anti-allergy, anti-inflamation and antioxidant activity was maceration in 95% ethanol.

Keywords: Anti-allergic activity, β-hexosaminidase, Anti-inflammatory activity, Nitric oxide, Antioxidant activity,
Prabchompoothaweep remedy, Extract