Background: Sa-Tri-Lhung-Klod is a Thai traditional medicine remedy for postpartum in the lists of The National Drug List of Herbal Medicine Products AD. It consists of seventeen herbs and were obtained by maceration and used in the form of liquor for women’s health care such as treatment of amenorrhea, menopause and blood tonic. In addition, it also used for postpartum care for being anti-inflammation in postpartum and prevention of cancer in women.
Objective: To investigate cytotoxic activity of Sa-Tri-Lhung-Klod remedy extracts and its herbal ingredients against human ovarian carcinoma cell line (SKOV-3) and cervical adenocarcinoma (HeLa) cell line.
Material and Method: Sa-Tri-Lhung-Klod remedy and its plant ingredients were extracted by maceration in 95% ethanol and dried using evaporator. All extracts were tested for cytotoxic activity by sulforhodamine B (SRB) assay.
Results: Ethanolic extract of Sa-Tri-Lhung-Klod remedy displayed cytotoxic activity against SKOV-3 and HeLa with IC50 values of 72.84+1.07 and 47.24+2.83 μg/ml, respectively. It was classified as “less-active” according to the NCI guideline. However, Caesalpinia sappan, Mammea siamensis and Curcuma comosa showed high cytotoxic activity against SKOV-3 with IC50 values of 9.55+1.38, 13.45+0.82 and 14.21+1.30 μg/ml, respectively. The ethanolic extracts of Caesalpinia sappan and Mammea siamensis also exhibited cytotoxic activity against HeLa with IC50 values of 6.30+0.06 and 7.72+0.11 μg/ml, respectively.
Conclusion: These results support the use of Sa-Tri-Lhung-Klod remedy in Thai traditional medicine for preventing of
ovarian cancer and cervical cancer. Caesalpinia sappan, Curcuma comosa and Mammea siamensis were strikingly active against ovarian and cervical cancer cells. Their extracts have the potential for developing as new anti-cancer drugs for women health.

Keywords: Sa-Tri-Lhung-Klod remedy, Cytotoxicity, Ovarian cancer, Cervical cancer