Objective: To determine accuracy rate of chromosome study from amniotic cellculture by coverslip technique compared with flask technique and to compared timing of amniotic cell culture, amount of amniotic cell culture media and cost of amniotic cell culture.
Study design: Cross sectional study.
Setting: Department of Obstetrics and Gynecology, Phramongkutklao Hospital.
Subjects: 70 pregnant women who underwent amniocentesis at Phramongkutklao Hospital during November 1, 2007 to February 29, 2008.
Intervention: Amniotic cell culture by flask technique and coverslip technique.
Main outcome measures: Accuracy of amniotic cell culture for chromosome study by coverslip technique compared with flask technique.
Results: Totally 70 pregnant women who underwent to amniocentesis and dividedamniotic fluid to cell culture by flask technique and coverslip technique. 69 samples had similar result from both techniques. The only one sample had cell culture failure inboth methods due to blood contamination. Accuracy in coverslip technique was 100% compared with flask technique. In timing of amniotic cell culture, amount of amniotic cell culture media and cost of amniotic cell culture between 2 methods that coverslip technique was lesser than flask technique.
Conclusion: There is statistically significant of accuracy in chromosome result between coverslip technique and flask
technique. Coverslip technique was lesser than flask technique in timing, amniotic cell culture media and costs of amniotic cell culture.

Keywords: Amniocentesis, Amniotic cell culture, Coverslip technique, Flask technique