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Original ArticleOpen Access
Low Cost Combination of DCIP and MCV Was Better Than That of DCIP and OF in the screening for Hemoglobin E
Prayongratana K 
,
Polprasert C ,
Raungrongmorakot K ,
Tatone K ,
Santiwatanakul S
,
Polprasert C ,
Raungrongmorakot K ,
Tatone K ,
Santiwatanakul S Objective: To evaluate hemoglobin E screening tests in a large scale of cases.
Material and Method: A cross-sectional descriptive study was conducted. Whole blood obtained from
subjects was evaluated for CBC, OF, DCIP, and hemoglobin typing.
Results: Five hundred twenty seven hemoglobin E and 280 reference subjects participated. DCIP’s sensitivity,
specificity, positive predictive value, and negative predictive value were 97.16%, 98.93%, 99.42%, and 95.19%,
respectively. These values of OF were 69.12%, 80.00%, 86.67%, and 57.88%, respectively. In the combination
of DCIP and OF gave rise to these values of 99.43%, 79.29%, 90.03%, and 96.67%, respectively. Finally the
combination of DCIP and MCV < 80 fL resulted in these values to be 99.43%, 98.93%, 99.43%, and 98.93%,
respectively. False positive and false negative rate were 1.07% and 0.57%, respectively.
Conclusion: Combination of DCIP and MCV was better than that of DCIP and OF in hemoglobin E screening.
Keywords: Thalassemia, Heterozygous hemoglobin E, Homozygous hemoglobin E, Dichlorophenolindolphenol
test (DCIP), Osmotic fragility test (OF), Mean corpuscular volume (MCV)
Material and Method: A cross-sectional descriptive study was conducted. Whole blood obtained from
subjects was evaluated for CBC, OF, DCIP, and hemoglobin typing.
Results: Five hundred twenty seven hemoglobin E and 280 reference subjects participated. DCIP’s sensitivity,
specificity, positive predictive value, and negative predictive value were 97.16%, 98.93%, 99.42%, and 95.19%,
respectively. These values of OF were 69.12%, 80.00%, 86.67%, and 57.88%, respectively. In the combination
of DCIP and OF gave rise to these values of 99.43%, 79.29%, 90.03%, and 96.67%, respectively. Finally the
combination of DCIP and MCV < 80 fL resulted in these values to be 99.43%, 98.93%, 99.43%, and 98.93%,
respectively. False positive and false negative rate were 1.07% and 0.57%, respectively.
Conclusion: Combination of DCIP and MCV was better than that of DCIP and OF in hemoglobin E screening.
Keywords: Thalassemia, Heterozygous hemoglobin E, Homozygous hemoglobin E, Dichlorophenolindolphenol
test (DCIP), Osmotic fragility test (OF), Mean corpuscular volume (MCV)
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