Background: Opisthorchis viverrini (OV) infection generates oxidative stress/free radicals and is considered as a primary
cause of cholangiocarcinoma since it primarily triggers sclerosing cholangitis.
Objective: In this study, the impacts of andrographolide on acute opisthorchaisis in β-naphthoflavone (BNF)-exposed
hamsters were investigated.
Material and Method: Ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) activities and
Thiobarbituric acid reaction substances (TBARS) assay of andrographolide in acute opisthorchiasis in the BNF-exposed
hamsters were assessed.
Results: The results showed that andrographolide ameliorated the hepatic CYP1A1 and CYP1A2 activities by decreases of the
specific enzymatic reactions of EROD and MROD, respectively, in the BNF-exposed hamsters. Moreover, andrographolide lowered the formation of malondialdehyde in the livers and brains of the hamsters.
Conclusion: These observations revealed the promising chemo-protective and antioxidant activities of andrographolide via
suppression of the specific EROD and MROD reactions and lipid peroxidation against acute opisthorchiasis in the BNFexposed
hamsters.

Keywords: Opisthorchiasis, Andrographolide, β-naphthoflavone, CYP1A, Lipid peroxidation, Hamster