J Med Assoc Thai 2016; 99 (11):118

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Effect of Curcumin on Collagen Deposition and Tissue Reorganization in Liver of Diabetic Rats
Yang-en S Mail, Petpiboolthai H , Khimmakthong W , Anupunpisit V

Objective: To localize and characterize type I and type IV collagens in recovery livers after curcumin supplementation in
streptozotocin-induced diabetic rats.
Material and Method: Induced diabetic rats were performed by streptozotocin injection (60 mg/kg BW). Male rats were organized into three groups, control rat (C), diabetic rat (DM) and diabetic rat supplemented with curcumin (DMC) (200 mg/
kg BW). At 8 weeks, animals were sacrificed. The localization and characterization of type I and type IV collagens in liver’s
cell and tissues were compared among C, DM and DMC groups by Sirius red and Immunohistochemical techniques, respectively.
Results: Type I and type IV collagens might be the key mediators of liver tissue healing associated with various disorders, especially with inflammation and reorganization processes. Concerning diabetic experiments, increased type I collagen was intensively recognized at subendothelial area of central veins whereas weakly demonstrated at periportal triad and perisinusoidal areas. Conversely, the high intensity of distribution of type IV collagen was strongly revealed at periportal triad and perisinusoidal areas while the intensity was faintly presented at central veins. In addition, accumulation of type IV collagen also revealed perisinusoidal basement membrane which was characteristic of capillarization of sinusoids. However, the localization of type I and type IV collagens was reduced after curcumin supplement in DMC rats compared with DM rats, implying that the liver tissue reorganization has been developed forwards to normal morphology. Moreover, type I and type IV collagen might distinctively accomplish the liver tissue reorganization by different means of area-based characterization.
Conclusion: The potential beneficial effect of curcumin has been exhibited the tissue reorganization of diabetic liver tissues.
The efficiency and achievement of curcumin might be applied to be an alternative therapeutic agent in diabetic hepatic pathology.

Keywords: Curcumin, Diabetes, Liver injury, Type I & IV collagens, Tissue reorganization

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