J Med Assoc Thai 2017; 100 (10):7

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Apoptosis Induction in Breast Cancer Cells by Cowanin
Chowchaikong N , Nilwarangoon S , Tanjapatkul N , Laphookhieo S , Watanapokasin R Mail

Background: Breast cancer is a common cancer occurs in women worldwide. In Thailand and United States, it is the first and second leading cause of cancer-related death among females, respectively. Therefore, a novel bioactive compound with high selectivity for cancer cell death is important in cancer research.

Objective: To investigate the effect of cowanin on anti-proliferation and apoptosis induction of breast cancer cell, MDA-MB-468.

Material and Method: MTT assay was used for cell proliferation and viability assay. Nuclear morphological changes and mitochondrial membrane potential was determined by Hoechst 33342 staining and JC-1 staining, respectively.

Results: Cowanin showed anti-proliferation and cell viability reduction in MDA-MB-468 cells in a time- and dose-dependent manner with IC50 value of 16.27±0.88 μg/mL. Furthermore, cowanin induced chromatin condensation, apoptotic bodies and mitochondrial membrane dysfunction in MDA-MB-468 cells.

Conclusion: Cowanin showed anti-proliferation and apoptosis induction in MDA-MB-468 cells. These results are basic knowledge for developing the chemotherapeutic agent for breast cancer in the future.

Keywords: Breast cancer cells, Cowanin, Apoptosis, Cells proliferation


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