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Original ArticleOpen Access
Chondrogenic Properties of Primary Human Chondrocytes Culture in Hyaluronic Acid Treated Gelatin Scaffold
Pruksakorn D 
,
Khamwaen N ,
Pothacharoen P ,
Arpornchayanon O ,
Rojanasthien S ,
Kongtawelert P
,
Khamwaen N ,
Pothacharoen P ,
Arpornchayanon O ,
Rojanasthien S ,
Kongtawelert P Objective: To study the possibility of primary human chondrocytes culture in gelatin scaffold and the effects of
exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix.
Material and Method: Cartilage tissue was engineered by using primary human chondrocytes with HAtreated
gelatin scaffolds and gelatin scaffolds. The chondrogenic properties were monitored for chondrocyte
proliferation, adhesion, and hyaline-like extracellular matrix production in both groups. The results were
compared to each other.
Results: Chondrocyte proliferation, adhesive activity, and new HA production were significantly increased in
HA-treated gelatin scaffold (p < 0.05). Immuno histochemistry for WF6 epitope demonstrated the higher
quality of hyaline-like extracellular matrix production. Moreover, the scanning electron micrograph showed
a higher filling of extracellular matrix in the pore of scaffold of HA-treated gelatin scaffold than that in non-
HA treated scaffold.
Conclusion: The present study demonstrated the possible role of commercial gelatin-based scaffold in
cartilage tissue engineering. It also demonstrated that exogenous HA-treated scaffold provides positive effects
for chondrocytes.
Keywords: Cartilage, Chondrocytes, Hyaluronic acid, Tissue engineering
exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix.
Material and Method: Cartilage tissue was engineered by using primary human chondrocytes with HAtreated
gelatin scaffolds and gelatin scaffolds. The chondrogenic properties were monitored for chondrocyte
proliferation, adhesion, and hyaline-like extracellular matrix production in both groups. The results were
compared to each other.
Results: Chondrocyte proliferation, adhesive activity, and new HA production were significantly increased in
HA-treated gelatin scaffold (p < 0.05). Immuno histochemistry for WF6 epitope demonstrated the higher
quality of hyaline-like extracellular matrix production. Moreover, the scanning electron micrograph showed
a higher filling of extracellular matrix in the pore of scaffold of HA-treated gelatin scaffold than that in non-
HA treated scaffold.
Conclusion: The present study demonstrated the possible role of commercial gelatin-based scaffold in
cartilage tissue engineering. It also demonstrated that exogenous HA-treated scaffold provides positive effects
for chondrocytes.
Keywords: Cartilage, Chondrocytes, Hyaluronic acid, Tissue engineering
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